Toxicity Of Phaeocystis Globosa On Six Marine Organisms

Objective: Phaeocystis as a harmful species could produce hemolytic toxin. Nowadays it was well known believed that the hemolytic toxin synthesized by many harmful algae might be responsible for the poison of marine organism. In this paper, the toxicity of Phaeocystis globosa on six marine organisms, Artemia sinica, Brachionus plicatilis, Moina mongolica, Epinephelus akaara, Lates calcarifer and Penaeus vannamei, were assessed and the toxic mechanism were discussed to provide the useful information on the ecological toxicity of P. globosa.Methods: The components of the hemolytic toxin from P. globosa in laboratory were analyzed by TLC and spraying a suspension of red blood cells (RBCs). The hemolytic activity was observed under a CKX41 inverted microscope. The toxic effects of the different components from P. globosa on Artemia sinica, Brachionus plicatilis, Moina mongolica, Epinephelus akaara, Lates calcarifer and Penaeus vannamei were assessed in the lab by semi-static water experimental method.Results: There were at least 4 components in the hemolytic toxin from P. globosa cultures in laboratory, which was slightly different from that of the wild P. globosa cells. Their Rf value was 0.73 (â… ), 0.39 (â…¡), 0.14 (â…¢), 0.04 (â…£) respectively and their hemolytic activity of component â… ,â…¡, â…¢, â…£, was 7.7,0,4.3,7 respectively.The results of toxicity test of P. globosa on the six kinds of marine organism were as follow: (1) Live P. globosa could affect the survival of larval Epinephelus akaara and Penaeus vannamei, whereas inhibit the motility of Artemia sinica or Brachionus plicatilis. (2) The re-suspended cells of P. globosa could inhibit the motility of Artemia sinica and Brachionus plicatilis. A lot of algal cells were found in their alimentary canal. This suggested that algal cells might have toxic effect on the zooplankton through alimentary canal. (3) The cell-free filtrate could affect the survival of larval Epinephelus akaara. However, the survival of larval fish was mainly dependent on yelk...