Construction Of Trichoderma Mutants By REMI Technique And The Mechanism On Bioremediation Of Cyanide Pollution In Soil And Water

Cyanide pollution causes great damage to agriculture production and ecosystems. Large quantities of cyanide are generated by metal plating, chemical, pharmaceuticals and synthetic fibers industry. To protect the environment from cyanide toxicity, the most commonly adopted methods are physical and chemical treatments. However, these methods are very expensive and most of all, they will cause second pollution. Thus biodegradation will be a potential way to degrade cyanide contamination in soil and water with the development of bio-remediation technique. Additionally, Trichoderma spp., as one of the most common biocontrol fungi, was considered to have cyanide degradation ability only the time it was symbiosis with plants. However, wild Trichoderma spp. used separately as cyanide degradation fungus was not efficient. Thus there is a need to construct Trichoderma mutants with higher cyanide degrading by bio-engineering techniques. Among those, REMI (restriction enzyme mediated DNA integration) was the most common method.REM(Irestriction enzyme mediated integration)technique was used to construct mutants from Trichoderma koningii T30, Trichoderma...