Study On The Character Of Cultivation And Reaction In Bioreactor By Gordonia.spWQ-01

Some corrosive damages, such as acid rain, have caused people's recognition on air sulfur pollution. The removal of sulfur in oil was one of the effective approaches to solve the problem of sulfur pollution. Hydrodesulfurization (HDS) is the dominating method to desulfurize oil, but it's defect of high operation cost and deep desulfurization limited the development of desulfurization technology. Biodesulfurization (BDS) which can selectively desulfurize the sulfur from the most recalcitrant under the mild conditions, remaining the combustion value of the fuels, support HDS very well. As more strict standard of sulfur content in oil appeared, BDS gain more attention.This article made strain Gordonia sp.WQ-01 to research on the level of reactor. First, the condition of high density culture was determined: 30℃,pH6.5,40%saturation of dissolved oxygen and initial substrate concentration of 0.25mmol/L. Under this condition, cell concentration could reach 4.26g/L as well as it's activity is 10.3μmol/g(DCW)h after 55h cultivation. The phosphate buffer which contains glucose, Mg²⁺,Ca²⁺ and Fe³⁺ was fit for the preparation of rest cells.Then strain Gordonia sp.WQ-01 was used to the two phase system composed of oil and water. It's degradation ability and reaction condition were investigated. Result showed that DBT of 1.7mmol/L could be degraded completely by Gordonia sp.WQ-01 in 16h when the ratio of oil-to-water was 1:5 , cell concentration in aqueous phase was 15g/L and stirred speed was 400r/min. Strain Gordonia sp.WQ-01 could be used to the degradation course of oil whose DBT content was lower than 6mmol/L.Finally, the effect of surfacetant on cell growth and degradation course was studied. 0.1%(v/v) of tween-20 had obvious effect on restraining the appearance of sugar cover outside of cell, which accelerated the transfer course of substrate and improved the efficiency of desulfurization.