Two Phase Of Immobilization Enzyme Acidfication And UASB For High Concentration Organic Wastewater Treatment

Up to now, the anaerobic treatment process is still play an important role in the high concentration with organic wastwater from food industrial. But there is not a practically feasible and reliable technique for the high temperature, high concentration, complicated wastewater containing protein from corn processing enterprise.The hard-degradation organic pollutant and the high-concentration sulfate are the most difficult problems in the wastewater.The high temperature, high concentration, complicated fractions starch wastewater from corn processing enterprise was treated as a subject in researching extreme temperature sludge domestication and culture technique as well as combining technology process, which was made up of innovative extreme temperature ( 70℃) hydrolyze acidification technology , immobilization acidification phase technology , extreme temperature two-phase anaerobic technology , high temperature aerobic technology and biological activity carbon(BAC).The thesis has revealed the physiological characteristic of the dominant bacterium lived in extreme temperature, change characteristic and mechanism of the factors that influence the acidification in extreme temperature, explained the treatment effect of the two-phase anaerobic reactor in extreme temperature , established the evaluating indicator system of effect on the extreme temperature acidification, and exploited an innovational treatment technology.The research was divided into three parts: the first part attached importance to study on the effect and mechanism of two-phase extreme temperature anaerobic treatment from the point of view of method, research on high temperature treatment and reusing technique of the wastewater from the point of view of the wastewater and primary discussed the assessment index system and evaluation method for hydrolysis acidification of the anaerobic treatment; the second part attached importance to study on the effect and mechanism of immobilization acidification and the mechanism of two-phase extreme temperature anaerobic treat.Protein-enzyme had been immobilized on big-hole resin ,activated charcoal and silica particles by a absorption method. This study focused on effect of enzyme immobilization, thermal stability and hydrolysis efficiency of wastewater. The result of batch test shown that the ratio of immobilized enzyme on activated charcoal, big-hole resin and silica particles was 61.95%, 37.85% and 20.31% respectively. The highest of thermal ability of activated charcoal, big-hole resin and silica particles was 18.18mg/min, 21.48mg/min and 3.6mg/min respectively at pH 5.5.The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times, The kind of amino-acid was increased from 14 to 16 kinds. The continuous- flow test result indicated that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor, The reactor was able to gain hydrolysis rate of 80% for protein and 36.1%(VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (about 500mg/L). The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively. The big-hole resin was a optimal carrier of using the immobilized protein enzyme of to hydrolyze protein particles in wastewater.as stated. above.Using immobilization enzyme acidification phase and UASB productuin methan phase as two-phase anaerobic fermentation process, the change characteristics and the change rate of organic acid and ethanol organic, composition and fermentation type as well as COD removal were studied. The experimental results showed that acidfication rate(VFA/COD) was consistently over 30% in acidfication-phase at a moderate C/N, protein hydrolysis rate was up to 95% and pH of enfluent was between 6.7 and 7.0; COD removal was over 91.3% at low C/N. The C/N of influent had significant effect on hydrolysis of acidfication phase and COD removal of methane phase. acidfication rate(VFA/COD) was consistently over 28.2% . The concentration of ethanol , acetic, butyric and propionic in acidfication-phase were 44.8%, 38.4%, 9.8% and 6.9%,respectively, as follow: ethanol> acetic>butyric >propionic. The removal of ethanol, acetic, butyric and propionic in methane production phase were 99.8%,92.0%,59.9% and 46.0%, respectively, and the removal of COD was higher than 90%. The conversion velocity of substrate in the methan production phase showed as follow: ethanol >acetic> butyric > propionic. The fermentation type of the acidification phase was ethanol-type fermentation. The effect of each acid and ehanol conversion on COD removal was as follow: ethanol>acetic> butyric > propionic.