| As an effective biological monitor to detect contaminants in the environment, the phyto-remediation technique based on accumulated plants is one of hot research field in the environmental around the world at present. In the thesis, the arsenic contaminated soils in Gap bases of the Chinese herb of Nephrolepis Auriculata Chuanxong, Dujiangyan City. It was supported by National Natural Science Foundation of China (40573044), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (2004527), and Applied Basic Research Program of Science and Technology Bureau of Sichuan Province (05JY029-088-1) to my supervisor.The main research contents are following:①arsenic speciation analysis by the resin separation-hydride generation-atomic fluorescent spectrometry;②study on the training of aqueous cultured N. auriculata(L.);③the choose of plant extraction liquid;④by the analysis of different forms of arsenic contained in the plotted Nephrolepis Auriculata, the enrichment and transformation behavors of arsenic in the accumulated plants are found; the analytical method of arsenic forms in the plants (biology sample) is established.The main solution in the study can be summarized as:①established the resin separation-hydrid-atomic fluorescent spectrometry analytical method of different forms of arsenic such as As(Ⅲ), As (Ⅴ), MMA, DMA;②the enrichment, migration and transformation rules of arsenic in the accumulated plants are found after analyzing different forms of arsenic such as As(Ⅲ), As (Ⅴ), MMA, and DMA with the analytical method of resin separation-hydride generation-atomic fluorescent spectrometry. And the result shows that the distribution feature is As(Ⅲ)＞As(Ⅴ)＞MMA＞DMA;③the remediation effect of Nephrolepis Auriculata to the contamination of arsenic is significantly obvious in basic condition when pH regulated, and adding EDTA or humic acid added appropriately. It was found that arsenic in the plants finally transformed into organic arsenic and stored in leaves in order to prevent the poisoning of plants. Therefore, this research can provide scientific foundation for the detoxification mechanism of accumulated plants. |
