Biodegradation Of Phenol Effluents By Immobilized Fusarium Sp.HJ01

Phenol and its derivatives are widely distributed due to theircommon presence in the effluents of many industrial processes, includingoil refineries, petrochemical plants, metallurgy, coal conversion precessesand phenolic resin industries. The application of immobilizationtechniques to phenols effluents has been the subject of numerousinvestigations by environmental workers. In this research, Fusarium HJ01has been applied to degrade phenol. The speciality of HJ01 with potentialfor phenol degradation and its biodegradation enzyme was investigated. Anew carrier—chitosan gel(CBC) was studied and partially characterizedphysically and chemically. The packed-column reactor filled with CBCgel-immobilized Fusarium HJ01 was constructed and operated to phenoltreatment.The main conclusions of the research are as follows: 1. Fusarium sp.HJ01 could grow using phenol as only carbonresource. Additional carbon can speed the phenol biodegradation. In thecondition of 3g·L^-1 sucrose, pH6, 30℃, 420mg·L^-1 of phenol was entirelydegraded within 6d. The maximum of initial phenol concentration whichthis strain can endure was 420mg·L^-1, when above the value, they wereinhibited.2. This strain can exhibited activity of both catechol 1,2-dioxygenase(C12O) and catechol 2,3-dioxygenase(C23O) in free cell extracts. Theactivities of C12O and C23O reach the highest value which were0.609U.mg^-1 and 0.125U.mg^-1 respectively when use phenol as singlecarbon resource. When C12O make function, activity was optimal atpH6.8, 40℃, while C23O has a higher activity in alkalescence conditionand was more stable at elevated temperatures when the optimal conditionis pH9.4, 60℃.3. The density and moisture rate of CBC gel are 1.00～1.07×10³kg·m^-3 and 13.00～14.50 respectively. Compared with Cu²⁺, it showsslightly influence on swelling rate of this cartier at the condition whentemperature changes from 10 to 80℃and pH from 4 to 10.4,In the condition of the same biomass and solid to liquid ratio, thecomparison of phenol degradation velocity are CBC gel-immobilizedby absorption＞Ca-alginate-immobilized＞CBC gel-immobilized byentrapment＞free cells. 5,In batch-recirculation mode experiment, the effect of air flow rateand phenol concentration to phenol removal is in accordance with CODremoval. The optimal air flow rate is 0.3mL·min^-1. After 32h, phenoldegradation efficiency and COD removal were almost 90% and 50% atthe initial phenol concentration 100mg·L^-1.6,The continuous flow process was started by the degradation of100mg·L^-1 phenol, when almost 90% phenol degradation was attained atthe optimal retention time 9.5h, when phenol concentration up to200mgoL-l, the phenol removal can reach 70%. This continuous flowequipment have a brightly application potential which greatly enhancedthe phenol degradation velocity compared to batch-recirculation system.