| Two parts are included in this thesis. In the first part of this thesis, thedeveloping history of Electrogenerated Chemiluminescent (ECL) and its type ofreaction are reviewed. In addition to these, the wildly analytical application of luminolECL system in the analytical chemistry are also given in this chapter.ECL is the luminescence generated by relaxation of exited state molecules that areproduced during an electrochemically initiated reaction in the near surface of theworking electrode. ECL is an important and powerful detection method in analyticalchemistry in recent years. So, this method not only retains the advantages over moreconventional CL. ECL analysis, compared with CL analysis, offered more alternativepotentials to regulate the ECL reaction for analytical purpose.Since the luminol-based ECL phenomenon was firstly observed by Harvey in 1929,the ECL was paid much attention by the analysts, and a lot of analytes had beendetected with luminal ECL system. ECL proved to be useful for analytical applicationand increasing investigations resulted in highly sensitive and selective detectionmethods. It has been applied successfully in many fields. Such as life science, clinicalmedicine, environment, immunoassays and DNA probe analysis etc.Recent years, the investigation of new analytical performances and analyticalapplication in luminol-based ECL system has been paid much attention by the analysts.In this thesis, we focused our interesting on the optimizing ECL performance of theluminol system by polyluminol compositing films modified electrode. The works areincluded in the followings:1. The Investigation of the Electrochemiluminescence Performances of theCompositing Films of Polyluminol with Metal Ions and its Analytical ApplicationIn this paper, it was found that the compositing films made from polyluminol andsome metal ions (such as Ni2+, Fe2+ and Co2+) can be easily generated on the surface ofthe graphite electrode by electropolymerizing luminol and these metal ions in the acidicmedium. Then, it was further found, compared to other resulting films, the compositingfilms made from polyluminol and Ni2+ presented the better electrochemiluminescence (ECL) analytical performance for urea based on its sensing effect for weak ECL signalof electro-oxidation polyluminol. Based on these findings, a novel ECL method for thedetermination of urea in urine samples was developed, and a new idea to improve theECL analytical performances of polyluminol by compositing metal ions was alsoproposed. Under the optimum experimental conditions, the ECL response regressedlinearly to the concentration of urea within the range from 2.0×10-9 g/mL to1.0×10-7g/mL with a detecting limit of 1.7×10-10g/mL.2. the Investigation of the Electrochemiluminescence Performances of theCompositing Films of Polyluminol with Organic Compound and its AnalyticalApplicationBased the electropolymerizing method, the compositing film of polyluminol andorganic compounds, can be fabricated on the graphite electrode surface. Then, it wasfurther found the resulting films can be enhanced by hydrogen peroxide strongly inalkaline solution. Based on these findings, a novel method for the determination ofhydrogen peroxide in rainwater was develop. Under the optimum experimentalconditions, the enhancing ECL intensity was linear with the hydrogen peroxideconcentration in the range of the 1.0×10-7mol/L to1.0×10-10mol/L (correlationcoefficient, 0.9970) and the detection limit for H2O2 was 6×1011mol/L.
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