The Regulation And Analytical Application Of Electrogenerated Chemiluminescence Reaction With Nanoparticles

Two parts are included in this thesis. In the first part of this thesis, the properties of nanoparticles and their analytical applications in luminescent analytical science are reviewed. In the second part, we mainly focused on the study of the synthesizing and analytical applications of core-shell luminol-doped SiO₂ nanoparticles and polyluminol nanoparticles in Electrogenerated chemiluminescence (ECL) system.ECL is the luminescence generated by relaxation of exited state molecules that are produced during an electrochemically initiated reaction in the near surface of the working electrode. ECL is an important and powerful detection method in analytical chemistry in recent years. So, this method not only retains the advantages of the conventional chemiluminescence (CL) analysis, but also offers some advantages over more conventional CL. ECL analysis, compared with CL analysis, offered more alternative potentials to regulate the ECL reaction for analytical purpose.Recent years, the preparations and synthesis of the nano-structured materials have attracted tremendous interest and a number of approaches for preparing the functionalized nanoparticles have been applied in the different fields of analytical chemistry. The purpose of this thesis was to regulate the reaction of luminol ECL system and improved their analytical performance by nano-technique.In this thesis, we focused our interesting on the synthesizing of the core-shell luminol-doped SiO₂ nanoparticles and polyluminol nanoparticles, applied it into luminol ECL analysis to regulate the reaction and improve the analytical performances of the conventional luminol ECL system. The works are included in the followings:1. A novel core-shell luminol-doped SiO₂ nanoparticle was synthesized by the two steps methods in microemulsion. While these nanoparticles were used as ECL reagent, the spatial regions of the electrochemical (EC) reaction as well as the subsequent CL reaction could be separated spatially and presented high sensitivity for determining isoniazid. Under the selected conditions, ECL response to isoniazid concentration was linear in the range of 1.0×10^-10 1.0×10^-6 g/mL and with 2×10^-11 g/mL detection limit.2. The core-shell luminol-doped SiO₂ nanoparticles were immobilized on the...