Study On The Trace Elements In Biologic Sample And Environmental Sample By Hydride Generation-Atomic Fluorescence Spectrometry

Atomic fluorescence spectrometry is a new instrument analysis method first appeared in 1960's.AFS has merits of high selectivity,high sensitivity and wide linear dynamic range .The hydride generation technology was used in AFS in 1970's. Recent years,As an special analysis way it has been widely used for determination of hydride-forming elements because of its high selectivity and sensitivity,wide linear dynamic range,fast speed of analysis and ease of use.Antimony and arsenic are a necessary trace elements for the health of people. In recent years, many investigations show that many diseases are related to intaking level of antimony and arsenic. Although many works have been done in the fields of determination, developing applied method to detect antimony in environment and body, appling techniques to prove up the biological behavior of different chemical and biological forms are significant.1. To establish new sensitive and reliable methods for determination of trace Antimony in Urine and Blood by Hydride Generation-Atomic Fluorescence Spectrometry. Using Hydrogen peroide (H₂O₂) and HNO₃ ,and a temperature control instrument to digest the sample ,and using HG-AFS to determinate Sb. In the given conditions, the linear range of determination of Sb is 0-50μg / L , the correlation coefficient(r) is above 0.9994,while detection limit is 0.15μg / L. The recovery is about 91.0%-106.5%. Not only is the new methods sensitive, the less sample volume, wider linear range, reliable and easy to operate, but also it benefits the operator's health.2. A new method was proposed for simultaneous determination of total arsenic and total antimony existed in the in Urine, Blood and environmental sample by hydride generation atomic fluorescence spectrometry (HGAFS) associated with an online flow injection system. The instrumental condition was optimized and the effects of the acid medium and the amount of reducing agent on the determination were investigated. The effects of analytical conditions and coexisting ions on the fluorescence signal intensity of analytes were investigated. The proposed method was provided with linear response ranges above 50μg /L for As and 50μg /L for Sb, and the detection limits of 0.67μg/Land 0.40μg/L were obtained for As and Sb respectively. The recoveries of 93.8-106.1% for As and 75.3-109.1% for Sb, and the precision of 0.79-2.22% and 1.16-2.69% (R.S.D, n = 3) respectively, were obtained via simultaneous determined some Urine, Blood and environmental samples successfully. The proposed method has the advantages of simple operation, high sensitivity and high efficiency.