The Spectrophotofluorimetry For The Determination Of The Coenzyme And Primary Amines Compound

CoA is a multiple nucleotide that contains pantothenic acid. It is natural coenzymes present in all animal and plant organisms. It is one of the most active metabolic compounds, and is involved in activation and acyl-group transfers in many enzymatic reactions. CoA plays an important role in the metabolism of carbohydrates, lipids and proteins. It obviously improves the symptoms of anorexia and spiritlessness, and in clinica it is usually used to treat leucocytopenia primary, thrombocytopenic purpura, functional low fever, and so on.Nicotinamide adenine dinucleotide phosphate (cozenmyⅡ) is a natural coenzyme present in all animal and plant organisms. It is essential to the function of many catabolic and biosynthetic pathways found within living organisms.It has a pyridine circle of nicotinamide, and using it oxygenation and deoxidization can occur reversibly, it can be used for the transfer of hydrogen in the metabolizability. Dysfunction of ATP production accompanied by a loss of cozenmyⅡdepletion can be observed, leading to the impairment of antioxidant mechanisms, loss of function, and finally to cell death.Histamine is one of the neurotransmitter which was confirmed in 80'times, The content of the histamine is high in the hypertrophy cells and its physiological activities such as contraction of smooth muscles, extension of capillaries and gastric acid secretions have been investigated. It is one of the chemical mediators of allergy and a neurotransmitter in mammalian brain, and it plays an improtant role in the systems of nerve, breath, cordis blood vessel, digest, immunity, internal secretion. Measurement of histamine in human urine is an important clinical parameter in various diseases such as bronchial asthma and atopic dermatitis, through immediate allergic reactions and cancer.5-hydroxytryptamine (5-HT) is well known as a neurotransmitter, present in most animal and plant organisms. It can improve the constringency, hyperplasia and migration of the blood vessel smooth muscle cell, activate blood platelets, induce the gathering of blood platelets, stimulate mitotic occurrence, activate T, B, NK and phagocytic hemocyte, participate in regulating the blood pressure and delivering nerve of the central nervous system. The increased content of 5-HT in human physiological fluids have been recognized in the patients with carcinoid syndrome, migraine and schizophrenia, so measurement the content of 5-HT in human urine is an important clinical parameter in various diseases and is an important basis in the research of mechanism of spirit paroxysm.In this paper, using molecule spectroscopy (fluorescence and UV spectroscopy) as research instrument, we study the interaction between drug-metal ion complex and biomolecule and the interaction between acetylacetone– formaldehyde and their application. There are four chapters.First, I summarized my thesis and introduced the investigation actuality of fremdness and homeland.Second, we provided a method to determine CoA using drug-metal ion as fluorescence probes and its application. In the Optimum buffer solution, CoA could remarkably enhance the fluorescence intensity of the drug-metal ion complex after adding H5IO6 and the enhanced fluorescence intensity is in proportion to the concentration of CoA. This method can be successfully applied to determination of CoA in injection, human serum and pig livers samples.Third, using tetracycline(TC) - europium ion (Eu3+) complex as a fluorescent probe, we studied the interaction between the complex and nicotinamide adenine dinucleotide phosphate (NADP) and its application. TC containingβ-diketonate configuration is a good ligand for Eu3+ and can transfer energy between TC and Eu3+ and the TC - Eu3+ binary complex can emit the characteristic fluorescence of Eu3+ at 612 nm. In the Optimum buffer solution, NADP could remarkably enhance the fluorescence intensity of the TC - Eu3+ binary complex after and the enhanced fluorescence intensity is in proportion to the concentration of NADP. In this part, using molecule spectroscopy (fluorescence and UV spectroscopy) as research instrument, we studied the fluorescence-increased effect of NADP on the complex system, explained the interaction mechanism. The developed method had been successfully applied to the determination of NADP in the synthetic water samples.Fourth, According Hantzsch reaction, primary amine can react with acetylacetone-formaldehyde to form a new ternary complex through cyclization and condensation reaction, the offspring which is N-substituent-2,6-dimethyl-3,5-dehydroacetic acid-1,4-dihydro pyridine can emit the greenish yellow fluorescence. The method has been successfully applied to the determination the content of histamine and 5-hydroxytryptamine in human urine and serum samples.