Application Of Solvent Sublation In Analysis Of Natural Product & Separation And Concentration Of Amino Acid

Solvent sublation was originally introduced by Sebba. In the process of solvent sublation, the hydrophobic compounds are levitated on bubble surface to the top of an aqueous column where they encounter a solvent layer to which the material is transferred as the bubbles move through the solvent layer. With its many advantages, such as simultaneous separation and concentration, little organic solvent used compared with solvent extraction and large water sample can be treated in short time, solvent sublation attracts much attention in the fields of environmental analysis, wastewater treatment and extraction of active constituents in natural products.In this paper, solvent sublation was successfully applied in two fields: (1) determination active components of natural product by solvent sublation followed by high performance liquid chromatography; (2) separation and concentration of amino acid by flotation complexation extraction.In the section one, pueraria, daidzin and daidzein in puerariae were separated and concentrated by solvent sublation, and effectively determinated by HPLC. The parameters of solvent sublation, such as gas flow rate, pH, sublation time, electrolyte (NaCl) etc. on the sublation efficiency were investigated in details. The optimal conditions of solvent sublation were also obtained. In the experiments of HPLC, the gradient elution and isometric elution of different ratio were compared. The limits of quantitation (LOQs) were 2.85 ng/ml for puerarin, 3.18 ng/ml for daidzin and 28.51 ng/ml for daidzein.In the section two, according to the advantage of solvent sublation and complexation extraction, the Flotation Complexation Extraction (FCE) was proposed, and the technique was used to separate and concentrate L-Phenylalanine from aqueous phase. The effect of pH, extraction solvent, electrolyte, concentration of P204 in organic phase, air flow rate and flotation time were investigated in detail. Under the optimal conditions, the L-Phenylalanine was separated and concentrated from aqueous phase, and the product was characterized by UV and FTIR. Moreover, the mechanism of Flotation Complexation Extraction was primarily discussed.