Screening And Applicated Studies In Kipper Process On The Repellent And Ovicidal Essential Oils For Flies

In recent years, with the increase of the ham production and sale volume, the traditional ham was just processed in the winter, but it has been altered into all year around, which caused the problem that the ham easily to be contaminated by flies in the summer and fall. It is always difficult to control flies because of lacking effective control technics and safety insecticides. Another traditional food, kipper, processed mainly in the summer is also facing the same problem. As the botanical metabolizing products, essential oils have the advantages, for example, nature, safety and no pollution on the enviroment etc. Many kinds of essential oil belong to traditional spice, codiment and food additive. In present, there are many reports on the essential oil used to control insect pests in home and abroad. A lot of essential oils have the good bioactivity of fummigation, contact toxicity and repellency against insects. The aim of this research is to develop safe and effective insecticides against flies through studying repellent and ovicidal effects of essential oils, so as to offer new effective technics for guranteeing cured food qulity.The contents of this research include:1. Tests on the ovicidal effect of over 30 kinds of essential oil on cured food pests, Lucilia sericata and Chrysomya megacephara. Cassial oil and its main component cinnamaldehyde were determined as the effective essential oil and component. Their LC₅₀ are 0.3687 mg. ml¹ and 0.7940 mg. ml^-1, respectively. GC/MS analysis results showed that the relative content of cinnamaldehyde, the main active component in cassial oil and synthesised cinnamaldehyde, are 95.43ï¼…and 94.90ï¼…, respectively.2. The optimized experiments of the emulsified cassia oil and cinnamaldehyde formulation. In order to fit the application of essential oils in food industry, effective ovicidal cassia oil and cinnamatdehyde, mixed with additive ingredients, Octulanddecylglycerate, sucrose ester and starch ester octenyl succinate were processed into water-soluble emulsion flavor by using the emulsification technics. The ovicidal test on the fly eggs placed dishes showed that both the optimum formular emulsified cassia oil and cinnamaldehyde had good ovicidal effects, their LC₅₀ are 0.8136 mg.ml¹ and 2.2836 mg. ml^-1, respectively. The ovicidal effects on the fly eggs placed on the kipper treated with 60 times solution of the optimum formular emulsified cassia oil mixture for 10 minutes, and 90 times solution of the optimum formular emulsified cinnamaldehyde for 5 minutes showed that the mortality of fly eggs reached 100ï¼….3. Experiments on the repellent effects of over 30 kinds of essential oils on Lucilia sericata. The repellent tested results showed that the repellency rate of the kippers soaked by essential oils from Ligusticum chuanxiong and Litsea cubeba in 6 hours at the concentration of 2.5μl. ml-1, reached 100ï¼…. According to GC/MS analysis results, some terpenes and terpene alcohols are considered the main active components.4. Food safety test on the emulsified cassia oil and cinnamaldehyde. HPLC analysis results showed there was little cinnamaldehyde residues in dried kipper soaked by emulsified cassia oil and cinnamaldehyde. According to acute toxicity tests, the emulsified cassia oil and cinnamaldehyde were accordant with the food criterion of actual no toxicity.