| Nowadays, there is a research upsurge of the functional food in the world. The prodectionof functional ligosaccharides has become a rising industry. The functional ligosaccharides havebeen a kind of important functional food additive because they have special physiologicalfunction. There will be a favorable development of preparation of functional ligosaccharidesvia biologic enzyme of material of wheat bran.The paper mainly studied xylan extraction from wheat bran, improvement of acquirementof xylooligosaccharides, refinement and function of xylooligosaccharides.The better conditions of removing starch was obtained:enzymatic quantity was120U/g, theratio of solid to liquid 1:12, temperature was 90℃and time was 40min. Protein's betterconditions: enzymatic quantity was 160U/g, the ratio of solid to liquid 1:15, temperature was40℃and time was 40min. The residue of starch was 1.62％and that of protein was 11.82％.Methods of ectraction of xylan were NaOH solution, combination of braising of acid andalkali, KOH solution and the combination of H2O2 and alkali. The variables which affectextraction:alkaline concentration, the ratio of solid to liquid, extraction time and temperatureand conditions of braising. The better conditions of NaOH solution: extraction temperaturewas85℃, the ratio of solid to liquid 1:12, extraction time was 2.5h, concentration of NaOHsolution was 2.5％and the content of xylan was 21.03％.The means of direct separation of enzyme and high-temperature braising were used. As aresult, the better conditions of enzyme: enzymatic quantity was 15U/g, pH 5.5, temperaturewas 55℃and time was 5h. And the content of xylooligosaccharides was 27.02％. Xylooligosaccharides from high-temperature braising was 36.31％: temperature was 150℃and time was 0.5h. Then residue separated of enzyme again, 34.19％ofxylooligosaccharides was obtained once more.Decoloration of xylooligosaccharides solution:active carbon, H2O2, D290. Pigment wasadsorbed 70％and lossing of xylooligosaccharides was 9％under 2％of D290, 50℃decolored3h.The methods of desalting were hyperfiltration and dialysis and the ratio of desalting were94.98％and96.57％respectively.Rats with different dose of xylooligosaccharides in functional experimentation wereaccelerated movement of intestine, shortened time of defecating, increased granule and weightof dejecta.Establish the former of high blood fat, rats of mid dose had the best effect in groups ofhigh, mid and low dose. And the three groups all had function of reducing blood fat. The comparison of blank and former in blood sugar explain the former of blood sugar wassucceed. And the high dose had remarkable function to rats.The rats' liver had swelled in comparative group. While the other three groupsdisappeared swelling. It was obvious that xylooligosaccharides had almost no harm on liver.This paper offered a new method for advancing xylooligosaccharides's preparation. Itcould deline the cost of xylooligosaccharides production and increase the value of wheat bran.It had good economic and social benefit.
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