Study Of Technology For Extracting Xylooligosaccharede From Wheat Bran

A xylooligosaccharede is composed of 2~7 xylose. It is formed after hydrolysis by the xylanase. It can promote the proliferation of Bifidobacterium, not digested, anti-caries, promote calcium absorption, improve symptoms of diabetes and improve the effectiveness of intestinal environment. The xylooligosaccharede market today is mostly sold by extracted from corn, The inadequate supply of xylooligosaccharede leading to high prices. The methods of extraction used at domestic and abroad most of acid or alkali, the step of purification is complicated. The corrosion of equipment is larger. The final product will be residual impurities. Given this situation, In this study, use wheat bran as raw material, exploring ways to efficient low-cost, high purity and low impurities in the production process, made the following research priorities:(1) Research on technology condition of remove the starch and peotein in wheat bran. It is used the wheat bran as raw material. Studied the best parameter of the ratio of liquid to solid,. the amount of amylase enzyme, hydrolysis time and hydrolysis temperature on removing the starch. Studied the best parameter of the ratio of liquid to solid, the amount of protease enzyme, hydrolysis time and hydrolysis temperature on removing the protein. It is used the iodine color determination to determine the residue of the starch. It is used the Kay-style set of protein determination to determine the residue of the protein. By orthogonal test to determine the best process parameters of separating starch from the wheat bran is that the ratio of liquid to solid is 1:20, the amount of the high temperatureα-amylase enzyme is 1200IU / g, the hydrolysis temperature is 70℃, the hydrolysis time is 40min. The verification test of the starch residue is 4.12mg / g. he best process parameters of separating protein from the wheat bran is that the ratio of liquid to solid is 1:25, the amount of the high temperatureα-amylase enzyme is 1000IU / g, the hydrolysis temperature is 50℃, the hydrolysis time is 5h. The verification test of the protein residue is 8.12mg/g.(2) Research on the ultrasonic with steaming process conditions of the extraction of the xylan from the wheat bron. It is used the wheat bran afrer separating the starch and protein as raw material. It is used the extraction rate as the detection. Studied the effect of the ratio of liquid to solid, the ultrasonic power, the ultrasonic frequency, the ultrasonic time, the steaming time and the steaming temperature. By orthogonal test to determine the best process parameters of extraction of xylan from the wheat bran is that the ratio of liquid to solid is 1:20, the ultrasonic power is 320w, the ultrasonic frequency is 70Hz, the ultrasonic time is 20min, the steaming time is 2h and the steaming temperature is 100℃. The extraction rate of the xylan is 60.27%.(3) Research on the extraction of the xylooligosaccharede with enzymatic preparation. It is used the crude xylan as raw material to hydrolysis with xylanase. It is used the average degree of polymerization xylooligosaccharede as detection. It is study the effect of the ratio of liquid to solid, the amount of the xylanase, the hydrolysis temperature and the hydrolysis time. By orthogonal test to determine the best process parameters is that the ratio of liquid to solid is 1:20, the amount of the xylanase is 1000IU / g, the hydrolysis temperature is 55℃, the hydrolysis time is 5h.(4)Rearch on the purification technology of xylooligosaccharede and product identification. Studied on decolorization medium such as activated carbon, PAC and macroporous resin. Finally, selection the resin D392 as the decolorization medium. Studied the influence of the ratio of resin, speed of agitator, the decolorizing time, the decolorizing temperature to decolorize the xylooligosaccharide. The result shows that when set the ratio of resin at 3%, speed of agitator at 140rpm/min, the decolorizing time at 3h, the decolorizing temperature at 50℃, the decoloration of xylo-oligosaccharide is 96.23%. The HPLC analysis showed that the peak time of after the initial purification xylooligosaccharide and the peak time standard xylooligosaccharide are same. The purification is 40%.