Determination Of Pesticide Residues In Milk

The extensive use of pesticide for agricultural and non-agriculture purposes has led to an increase in public concern about possible health risks due to toxicity through exposure to pesticides. As a daily food, safety of milk is very important. To assure milk safety, quick, efficient, sensitive methods to detect pesticides residue in milk are needed. In this paper, the present state and recent progress in analytical technologies of pesticide residues in food and dairy products are summarized, and experiments were carried out to establish different methods for detection of different pesticide residue in milk.For methods based on GC, extraction of pesticides with solvents, and the parameters of Gas chromatography were studied. All pesticides were identified by retention time, and quantified by extra-standard. Accuracy, precision and the limits of detection (LOD) of the method was validated. Analytical method for organophosphorus, organochlorine has been developed to determine pesticide residues in milk.Methods to detect pesticide residues in milk based on enzyme inhibition have been studied. The toxic action of organophosphate and carbamate pesticides is based on the rapid irreversible inhibition of the cholinesterase (ChE), so the pesticide residues in milk can be detected by analyzing the change of ChE activity. On the basis of this principle, two rapid detection methods with colorimetry and rapid test paper for pesticide residues were developed. Among the pesticides detected, sulfureous organophosphates had lower inhibitory ability, but phosphorothionate pesticides could be detected after direct oxidation with N-bromosuccinimide.Rodenticide is a kind of pesticides to kill murine. It is a major source to revoke acute toxicity. This paper studied chemical methods for detecting rodenticides in milk, and developed quick methods to determine rodenticide, includingα-Naphthylthiourea , Diphacinone.