Study On Preparative Technology For Laliao Granula

Laliao herbs belongs to Polygonaceae plants, drying entire plants of Polygonum Hydropiper L. and Polygonum Flaccidum Meisn. It has difference names,such as water pepper,flaccid knotweed herb,urin- herbs. Mostly be founded on the edge of the river or the wet land of slope of hill. It has many places of produce. The traditional medicine study has confirmed,Laliao herbs has the nature of acrid,mild, innocuity, and invigorating spleen to eliminate dampness, dehumiditing and eliminating stasis,stopping dysenteria and abdominal pain,disintoxicating and eliminating. It applys to diarrhea, abdominal pain, dysenteria, which result from infestation or bad food and drink. The modern medicine study has confirmed,the total flavonoids of Laliao herbs has the nature of antibiosis, anti-virus,anti-inflammatory,antioxygen,hematischesis,anti- tumor,and relieving pains. The flavone is active component.Laliao herbs are widely used in Hunan province and Hupeh province,mainly can cure diarrhea, enteronitis dysenteria, dysenteria,and have good therapeutic effect. Based on this traditional popular prescription,we have a idea about researching Laliao patent medicine.《Bie Lu》say"leafs of Laliao, belongs to meridian and vessels of tongue,eliminates evil factor affecting health,benefits people's bodies". The modern medicine study has confirmed,Laliao apozems have significant depressant effect to bacterium dysenteriae,bacillus diphtheriae, bacillus proteus,bacillus typhi murium,bacillus aeruginosus,bacterium coli.So it can be used to therapy diarrhea,gastroenterocolitis,dysenteria. Laliao have warm-natured. Li Shizhen's《Compendium of Materia Medica》has recorded"leafs of Laliao have the nature of acrid,warm and detoxication".So Laliao can hold the post of the principal drug,that activate spleen-energy,dehumiditing and eliminating stasis,so that the spleen and stomach have good transportation and transformation, have distinguished pure and turbid, and have elimated intestine pathogenic damp.At the same times,it have possess deintoxication,promoting blood flow, alleviating pains,so that it can cure many kinds of intestine disease and other concurrent disease resulted from long time diarrhea. In one word, Laliao, false indigo herb, have reflected simultaneous treatment of principal and subordinate symptoms,that mainly in invigorating spleen to eliminate dampness, less in promoting blood flow and alleviating pains.The modern medicine reseach shows that it regulate extreme hypofunction of the intestine and stomach and immunologic function.ObjectiveIn order to exploit whether rutin exists and establish a method for the content determination of total flavonoids in Laliao. To check the optimum extraction technology and suitable excipients. To select better formalizing process of Laliao Granule.MethodsThe Identification of Rutin in Laliao by TLC and UV: Physicochemical Properties and assaying of Laliao was identified by the methods of thin-layer chromatography (TLC),ultra-violet spectrophotometry (UV).The optimum extraction technology of Laliao's verification test: To verify optimum extraction technology: The index is yields of extracted powder using orthogonality design observation,discern investigation the relationship with dosage liquor ratio,abstraction time,abstraction frequency.To select the suitable excipients: To select the suitable excipients from lactose,starch,dextrine,and investigating moldability,hydroscopicity,angle of repose,and fluidity.Preliminary study on formalizing process of Laliao granula : To select the optimum formalizing process of Laliao granule: The index is granularity using orthogonality design observation,and investigating the ratio of the extracted powder and excipients,alcoholic concentration,drying temperature.ResultsThe Identification of Rutin in Laliao by TLC and UV: Rutin was mainly active component in Laliao. Good linear relationship with the absorbability was achieved when the detection concentration of rutin was within a range of 0.00808-0.04848mg/ml (r=0.9928),the regression equation was A=11.4716C- 0.0003 (r=0.9928,n=6) and the average recovery was 97.95%(RSD =1.88%). Meanwhile,the average content of total flavonoids was 1.939% in Laliao sample.The optimum extraction technology of Laliao's verification test: The analysis of variance shows that factor A (abstraction frequency) has noticeable effects,factor C(the ratio of the dosage liquor)is no more than factor A,and has not apparent effects,factor B(abstraction time) has hardly any effects. the optimum extraction technology of Laliao: the ratio of the dosage liquor is one to ten,decocting forty minutes every time with waters,totally three times.To select the suitable excipients: Through the synthetic evaluation,the moldability,hydroscopicity,angle of repose,and fluidity of three excipients (lactose,corn starch,dextrine),we can select the suitable excipients to make Laliao granula. The results shows that the combined excipients of corn starch and lactose are better.Preliminary study on formalizing process of Laliao granula: The better formalizing process: the ratio of the extracted powder and corn starch and lactose was 2:2:1,the wetting agent was 75% alcohol and the drying temperature was 70℃. The Laliao granule had better fluidity and critical relative humidity(CR H)was about75%.ConclusionThrough the technology investigation, this method that the content determination of total flavonoids in Laliao,is simple, rapid,and accurate.Good linear relationship with the absorbability was achieved when the detection concentration of rutin was within a range of 0.00808-0.04848mg/ml (r=0.9928),the results are accurate.The optimum extraction technology of Laliao: the ratio of the dosage liquor is one to ten, decocting forty minutes every time with waters, totally three times. With the critical relative humidity(CRH) is about75%, the index is granularity of granula using orthogonality design observation,the granula have very good quality control. Based on above factors,we can decide a reasonable preparation technology line about Laliao granula. And it provides datas for the exploitation and quality criteria of Laliao granula research. .