The Research On Extraction Technology Of Pigment,Total Flavonoids And Total Polysaccharides In Lycium Chinese

The Lycium chinensis for medicinal purposes part is the fruit of the Solanaceae the (Lycieae L) piant.New pharmacological constantly being discovered,Modern medical studies have shown:wolfberry have immunomodulatory,antioxidant, anti-aging, anti-tumor, anti-fatigue,lowering blood sugar, lowering blood pressure, kidney, liver, eyesight, resistance to radiation damage and other effects.The demand of the Lycium chinense increases suddenly day after day,causes artificial planting medlar to be valued day by day.The people have proposed the new request on the quality of the Planting wolfberry. Wolfberry supplied by Jing tai,Bai yin is the object of study for this topic,experiment in researching pigment ultrasonic-assisted extraction process;Total flavonoids microwave extraction process; Total polysaccharides microwave extraction process;Purification of flavonoids extract Lycium.The experiment establishes the best comprehensive utilization process of the wolfberry.(1)The optimum extraction process of ultrasonic-assisted is selected with the orthogonal design.The yield of pigment were used to evaluate the factor levels.The exttaction process is:(Petroleum ether+chloroform),temperature,55℃,the ratio of solid to liquid,1:8;Ultrasonic power,160w and the return of ultrasonic-assisted 40min; The process of total carotenoids extract,guarantee a highter content,and optimize Lyceum barbarum L total carotenoids the extraction process,Wolfberry color pigments reach to more than the value of 129.(2) The orthogonal design was used in the research on the extraction technique of total flavonoids from LyceumbarbarumL.with organic solvent assisted with microwave.The optimum conditions on the extraction of total flavonoids from Lyceum barbarumL.was as follows:ethanol:96%;the ratio of solid to liquid,1:12; Microwave power 400W;The return of microwave-assisted,40min.Achieve wolfberry extract flavonoids yield 62.72%,total flavonoids from dry extract up to 2.69%.The technique ensured a high content of flavonoids extracted, which was the optimization extraction measure of total flavonoids from LyceumbarbarumL. residue.(3) Six kinds of compounds were isolated from flavonoids extract,in which,five kinds of compounds were identified by chemical and spectral methods,they are tetracosane,β-carotene,β-sitosterol,Acacetin,luteolin,Rutin. (4)The orthogonal design was used in the research on the extraction technique of total polysaccharides from LyceumbarbarumL.with wather assisted with microwave. The optimum conditions on the extraction of total polysaccharides from Lyceum barbarumL.was as follows:wather;The ratio of solid to liquid,l:12;Microwave power 500W;The return of microwave-assisted,40min.Achieve Lyceum barbarumL extract polysaccharides yield 13.56%,total polysaccharides from dry extract up to 55.35%. The technique ensured a high content of polysaccharides extracted, which was the optimization extraction measure of total polysaccharides from LyceumbarbarumL. residue.