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The Research On Way Of Biology-dewatering And Moisture Character About Dejecta Of Domestic Animals

Posted on:2008-01-20Degree:MasterType:Thesis
Country:ChinaCandidate:H Y FeiFull Text:PDF
GTID:2121360242465566Subject:Environmental Engineering
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Livestock manure emissions is serious pollution to the environment. Aerobic fermentation can be used to solve pollution problems caused by faeces widely. However, the test results have shown that 50% -60% of normal moisture content suitable for composting. Non-washing manure moisture is generally 60%~85%. Manure moisture content should be regulated to the extent appropriate with a certain degree of difficulty. In particular, because of resource and cost conditions, measure of moisture opsonin can not be used. Although the use of mechanically separated completely basic separation of solid-liquid separation, greater energy consumption. Therefore, the study of techno-dehydration of animal wastes is very important for the fermentation.In this study, We analysed quality about moisture content characteristic, shape of solid. etc for the fresh sample of cow manure, pig manure, chicken manure and so on. The result illustrated that sample of cow manure per gram have the best ability of drink to water, to 7.14g·g-1. The proportion of absorb water with the capillary shape account for 61.8%; but pig manure and chicken manure are merely up to 3.36g·g-1, 4.62g·g-1 respectively. The content of fiber and colloid are obviously higher than pig manure and chicken manure, it is about 51.6%, 3.4% respectively, CECv of cow manure is about 28.74milligram·100g-1. For the determination moisture content characteristic curve, we carried on fibre-enzyme processing(lower fibre), the acid treatment(transform CECv), removes the colloid. The result indicated the textile fiber content, the colloid content and the colloid surface negative charge and the infiltration capacity have the certain relevance. There have further studies how to destroys the structure of stores water which is made up of textile fiber and colloid forms.etc and transfers which will not be easy absorbed water, capillary water from which will easily to remove the gravitational water, it offers theory of bio-dehydration.In this study, we used the 10%FeCl3 solution, the 10%NaOH solution, 1%polyacrylamide solution to the cow dung in (freezing preservation, when use dilution is 1‰), 10% H2SO4, 20% SDS, 20% DTAC, 20% Tween, experiments its solid fluid separation effect. The result indicated that, after the acid and the surface active agent processing cow dung, the solid fluid separates the effect to be best. Because producing the surface active agent microorganism need hydrocarbon compound physical existence, the good oxygen condition, can produce the surface-active substance, is not feasible in the actual condition. Therefore we choose the study of change pH and destroy fibre-sluice framework, expanded new more research.This research has screened the nature universal Lactobacillus, it's Lactobacillus.sp after the preliminary appraisal; Simultaneously aims at in the cow dung the textile fiber (to see second chapter) in moisture content characteristic importance, after ameliorated PCS culture medium, a cellulose degradation microbial community Xc was isolated from sullage, soil of forest, cow manure and haulm of compost, through change method of inoculation. It was found that 81% of filter paper, 41% of absorbent cotton, 25% of wheat straw, or 40% of fibre of cow manure can be degraded by 100ml of Xc at 37℃with in 120 hour under static culture. At 37℃and 28℃, the XC saccharification activity were much higher at 120,168th hours when degrading filter paper that were 104.5U·ml-1, 95.3 U·ml-1. The Xc could be inoculated in a wide pH rang, from 5 to 9; however, the final pH would be changed to neutrality after incubation and have efficient ability. This capability of degradation maintained more than 22 days when the substrates were continually added. The XC could be inoculated in 30 days. The pH would be stability with filter paper between 6.5 and 7.5, and about 8.5 with out the filter paper, The main DNA bands are not changed by the method of 16SrDNA PCR-DGGE after culture of five months so that Xc is very stable.The use of cow dung screening agent for the study of solid-liquid separation. The cow dung were obviously out of the water chromatography phenomenon. It dicated that pH agents were quickly dropped the pH of cow dung. 6% level were mixed with paragraph 10 days has reached the lowest pH value of 5.31. finally to 5.15 in 30th days, below the 5.95 CK. The pH of cow dung mixed rapid decline in vaccination coverage of a cow agent, the reason could be that some fiber degradated, microbial breeding more easily, produce more organic acids. Effect of microbial inoculum of solid-liquid separation has been better than cow dung CK. 6% level were mixed solid-liquid separation clause after 15 days has reached the lowest water rates to 78.3%. in 30th day to 77.7%, below the 82.1% CK. Solid-liquid separation of cow dung mixed results were better than single inoculation of cow dung. The reason could be that Xc degradated fiber, damaged the water-structure, so it release more free water. After cow dung inoculumed microbial, evaporation were 74.2g in 6% level, evaporation of CK was 39.2g, the reasons for the increase of free water content, crude fiber of cow dung were decreased. In Xc 6% level of inoculation, Crude fiber content of cow dung was 45.6%, lower than the 52.30% of CK. The experiment indicated two kind of microbial inoculum caneffectively enhance the cow dung dehydration degree.
Keywords/Search Tags:Livestock manure, Moisture characteristic, Bio-dehydration, Cellulose degradation, Solidus-liquidus segregation
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