| The determination of amino acid has significant meaning in both theory field and application field. Nowadays there are various methods of amino acid determination, including chemical analysis, spectrum analysis, electrical analysis and chromatography analysis. However, it is rare to use multivariate calibration to measure amino acid. In this thesis, the determination of single component amino acid and double components mixed amino acid is carried out through multivariate calibration titration with solution of NaOH or HC1 as the titrant, hydrogen ion selective electrode as the indicator electrode, while automatic titrator is operate instrument. Hereby, following jobs have been done: (1) The best titration way for determination of each single acidic, alkalescent, neutral amino acid component and its various double mixed components is summarized through reviewing the four titration ways, including direct titration with acid, direct titration with alkali, back titration with acid and back titration with alkali. (2) Through the best way of titration, desirable determination outcome of Glu, His, Arg and Ser single component and its mixed double components is obtained, the errors is about 1% for single component while about 3%for double components. (3) Each titration curve of single amino acid component and double mixed components, obtained from every titration way, is systematically discussed and explained.
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