Effects Of BaP Exposure On Immunity Functions Of Sebastiscus Marmoratus

In this study, the immune reactions of Sebastiscus marmoratus were examined, after exposure to 0.1, 1.0 and 10.0mg/kg BW of BaP for 1, 4, 7 and 14 days. Exposures were conducted with peritoneal injection. The immune parameters of this study including immune organs, immune cells and immune moleculars.The objective of this study was to investigate the immune functions of Sebastiscus marmoratus after exposure to BaP, to investigate whether sublethal concentration of BaP exposure can injury the immune functions of Sebastiscus marmoratus, to find sensitive biomarkers in the immune parameters and whether the immune system of Sebastiscus marmoratus can be a sensitive model to monitore the exposure of BaP.1,Effects of BaP exposure on immunity organs in Sebastiscus marmoratus were detected. The results showed that BaP strongly induced spleen weight/body weight after exposure for 1 day, and then Restored to the control level. And Bap induced the liver weight/body weight significantly after exposure for 4 days, but it was inhibited after exposure for 14 days. The results revealed that a short time Bap exposure induced the immune organ hyperplasia , but a long time BaP exposure will lead to immune organ damage and atrophy of Sebastiscus marmoratus. Spleen appeared to have a strong organizational repair capacity.2,Effects of BaP exposure on immunity cells in Sebastiscus marmoratus were detected. The spleen lymphocyte proliferation of Sebastiscus marmoratus was very sensitive to BaP exposure, which revealed that spleen lymphocyte proliferation was a susceptibility biomarker; There were good dose-effect and time-efect relationship between Sebastiscus marmoratus's blood phagocytic cells respiratory burst and BaP concentrations, which revealed that blood phagocytic cells respiratory burst was a effective biomarker.Experimental results showed that BaP exposure inhibited Sebastiscus marmoratus's blood red cells number and the number of blood white cells were induced then turned to be inhibited, which showed that BaP exposure in a relatively short period of time-induced leukocyte number, however, a longer exposure time will impair the Sebastiscus marmoratus's hematopoietic tissue, and then to suppress immune functions.3,Effect of BaP exposure on immunity enzymes in Sebastiscus marmoratus were detected. BaP exposure on Liver SOD activity of Sebastiscus marmoratus finally represented inhibition; And BaP exposure on Liver AKP and ACP all represented strong inducement. SOD activity decreased showed the damage of Sebastiscus marmoratus's antioxidant; And AKP and ACP activity increased might imply the carcinogenesis of Sebastiscus marmoratus.In summary, the immune functions of Sebastiscus marmoratus inhibited after BaP exposure; Spleen lymphocyte proliferation and phagocytic blood cells respiratory burst of Sebastiscus marmoratus were possible as sensitive biomarkers to monitor BaP exposure; Liver AKP and ACP activity increased may be the precursor of the carcinogenesis in Sebastiscus marmoratus.