Application Of Dietary Fiber-based Microencapsules From Wheat Bran In Improving The Survival Of Lactobacillus Acidophilus NCFM

Probiotics in extreme environmental conditions of survival is very low, a combination of dietary fiber microencapsulation technology to embedded probiotics not only improve the probiotics in adverse environmental conditions of survival, and that dietary fiber and the physiological function of probiotics Characteristics of combining to form a nutrient-rich additives.This paper studies the technologic process of extracting dietary fiber from wheat bran, and find the best technics,whith is to use 0.5% amount of extract– amylase to hydrolyze dietary fiber and then use 1% concentration of NaOH alkali solution 100 min, alkali solution at temperature of 60℃for 100minutes.I made the microcapsule with the Lactobacillus acidophilus solution and the soluble dietary fiber as a core material, insoluble dietary fiber and sodium alginate, made of gelatin as a microcapsule wall material. the collapse of tolerance test show that wall material (not insoluble dietary fiber 0.5 g +3% gelatin: 2% sodium alginate: = 1:1): core material (50% of soluble dietary fiber: Lactobacillus acidophilus bacilli = 1:1) by made of the ratio of 1:1 compose the best microcapsule.Microencapsulation of L. acidophilus strain NCFM subjected to extreme environments including gastric juice, intestinal juice, acidity condition, pepsin solution, trypsin solution, bile salt and NaCl solution was also studied. The results showed that microencapsules containing the cells of strain NCFM were completely broken out in 70 min, and viable cell counts released reached 2.97×107cfu/g. The viability of the microencapsulated strain NCFM treated in pH 2.5 was significantly improved compared to the control (P<0.01). The viable cell counts of microencapsulated cells were 1.08×108cfu/mL, whereas those of the control were 2.7×107cfu/mL.The artificial stimulation of gastric and intestinal environments to treat microencapsulated strain NCFM for 3h and 24h showed that the viable counts of microencapsulated cells was significantly higher than the control, reaching 5.44×106cfu/mL and 2.57×106cfu/mL respectively, whereas the control was only 3.41×106cfu/mL and 1.35×106cfu/mL. Exposure strain NCFM microencapsulated to 14g/L pepsin solution showed that compared to the non-microencapsulated cells, the viable cell counts of micro-encapsulated cells were significantly improved, up to 3.5×106cfu/mL. In contrast, the viable cell counts of the control treatment were 0.85×106cfu/mL.When treated in 0.2% bile salt, the viable counts of microencapsulated cells were higher than those of the control (P<0.01), reaching 2.1×106cfu/mL. The live cells of the control were 0.95×106cfu/mL.Exposure of strain NCFM microencapsulated to 3g/L trypsin solution indicated that compared to the non-microencapsulated cells which were 0.68×107cfu/mL as the control, the viable counts of micro-encapsulated cells were 5.35×107cfu/mL.When treated in 18% NaCl solution, the viable counts of microencapsulated cells were up to 1.1×108cfu/mL, higher than those of the control which was 1.2×107cfu/mL (P<0.01).In short, it was concluded that use of microencapsulation presented in our study to treat L. acidophilus promoted the survival of this organism subjected to extreme environments.