Study On Transesterification Of Woody Oil To Produce Biodiesel By Immobilized Lipase

Biodiesel has appealing more people as an alternative to diesel fuels made from vegetable oils, animal fats or used frying oils. Biodiesel is renewable, biodegradable and environmentally benign. The materials used in this paper were Lindera Communis Hemsl(LCH) seed oil and Pistacia Chinensis Bunge (PCB) seed oil, the qualitative and quantitative composition of the oils and enzymatic ayntheses of biodiesel via LCH and PCB oils were studied. The performance of biodiesel had been evaluated.The qualitative and quantitative composition of LCH and PCB: The acid value of LCH seed oil was 1.19mgKOH/g, saponification value 215.5mgKOH/g, the mainly composition of oil was saturated fatty acid(Capric acid 33.48%, Lauric acid 59.14%); The acid value of PCB seed oil was 18.03mgKOH/g, saponification value 155.3mgKOH/g, the mainly composition of oil was unsaturated fatty acid(Linoleic acid 34.43%, Octadecenoic acid 46.77%).Study was carried out on gradient elution reversed-phase high-performance liquid chromatography (RP-HPLC), it was used for the determination of compounds occurring during the production of biodiesel from oil.Reservoir A contained methanol, reservoir B congtained 2-propanol-hexane (5:4,v/v). Builded six linear regression equations of fatty acid standards. A linear gradient from 100%A to 50%A +50%B in 15min. It was used for fast monitoring of conversion of the oil triacylglycerols to fatty acid methyl esters and for quantitation of residual TGs in the final biodiesel product.The optimized parameters to catalyse LCH seed oil to biodiesel by the immobilized lipase were made by the Response Surface Methodology, tert-butanol was adopted as a reaction medium for lipase-catalyzed methanolysis of oils for biodiesel production, Lipozyme TLIM was used as catalyst, the biodiesel with best conversion 90% was produced at the reaction temperature, 38.5℃; the ratio of mole quantity between methanol and oil, 4; the ratio of volume between tert-butanol and oil, 1; Lipozyme TLIM catalyst concentration, 4%.The biodiesel of LCH and PCB oil had the fuel properties such as density, kinematic viscosity, flash point, pour point, cold filter plugging point, cetane index, distillation temperature and fatty acid composition within the limits prescribed by the American (ASTM)standards, except for a slightly lower flash point(115℃)of LCH biodiesel and a higher kinematic viscosity(6.42mm2/s) of PCB biodiesel than that prescribed by the latter standard.