Research On Equipment Of Continuous Chromatography Intergrated Separation And Reaction For Chitosan-oligosaccharide

Chitosan-oligosaccharide(COS)is a linear polymers,in which 2-10 D-glucosamine residues are connected byβ-1,4 linkages,which has unique physiological and biological activities,especially when the polymerization degree is 6,it plays a important role in the field of anti-tumor,thus,it finds a vast application in medicine science,environmental protection and even agriculture.COS is degraded from chitosan through kinds of methods such as bio- fermentation process, chemical discomposition process and enzyone method.Usually,the products deprived from above processes are mixtures compounding with a sery of Dglucosamines with different polymerization degrees,and in order to select the very chitin with a polymerization degree of 6,(to be simplification,we call it 6-COS),a device named Continuous Chromatography Intergrated Separation and Reaction Device,under the idea of the patent:Methods and Practical Devices of The shunt multiple separation intergrated with reaction was developed.With its help,this article researches into the characteristics and methods of 6-COS's multiple components intergrated separation,from the avenues of practical experimentation and numerical simulation,eventually,gains a rather good effect of practical application.First,according to the characteristics of the mass transfer between the bulk phase and the particle phase during the separation process,equations can be established separately in the bulk phase and the particle phase,with the absorption isotherm,the initial conditions and boundary conditions,those mass transfer equations can be solved,adopting the Matlab' s ODE solver.The results will be displayed in the forms of historic concentration curves inside the column and the real-time concentration profiles at the outlet of the column.Those concentration profiles will provide guides for the researches of Chromatographic Separation.Second,installed a device named Continuous Chromatography Intergrated Separation and Reaction Device,under the idea of patent:Methods and Practical Devices of The shunt multiple separation intergrated with reaction,and executed practical experimentation researches,gained the best point of PH control and the best switching time for the system of 6-COS's separation,those results provide pre-conditions for more complex separation processes.Besides,this article got the absorption isotherms of 6-COS,under the temperature of 20 degree,when the absorbent was C115E,and bed's porosity via the method of experiments.All above make it possible to install the model of the chromatographic separation process,and get the simulated results.At last,after solving the model mass transfer equation,COS's concentration profiles inside the column and breakthrough curves will be attained.Those curves have good conformations to the curves derived from practical experimentations. These conformations are proves of the effectivity of the model solutions methods. Then,more researches are developed about the influencing factors to the chromatographic separation progress,focused on the axial dispersion coefficient, bed void porosity,column length,switch time and flow rate in different zones.All those researches provides modeling guides for determining the best operation conditions and installing new chromatographic separation systems.