The Craft Research Of Ultrasonic Method And Enzymatic Hydrolysis Method Union Separates The Goose Serum Globulin

The goose blood resources is rich, is the precious animal protein resources, the goose blood protein content reaches as high as 23%-28%, globin, transfers ferroprotein, the immunity globulin and so on all is has the biological activity function the protein, the blood corpuscle protein content reaches as high as 45%, mainly by the hemoglobin constitution, the content accounts for the blood corpuscle protein total quantity above 90%, is in the blood the protein mainly gathers is at. However, because in the goose blood fishy smell and the blood red pigment, have limited to the goose blood resources use, the thorough research is extremely deficient, the comprehensive utilization also therefore is restrictedv.Eliminates rank to the domesticated fowl class animal blood decolorization, must unify the highly effective cell break method and the decolorization method, in the decolorization simultaneously the enhancement production effect, reduces the protein loss factor. At present domestic and foreign includes to the animal blood broken hematolysis method: The enzyme dissolves the law, chemistry osmosis process, swelling method, the machinery break method and so on, but these methods have cost high, some existence chemical reagent remain, some consume when is long, the production efficiency is not high; The decolorization method mainly has: oxidation method, machinery method, high-molecular compound flocculation law, organic solvent extraction, but some decolorization effects are not ideal, some production costs are high, some organic solvents remain the with difficulty separation, is disadvantageous to the animal blood decolorization.This article has compared the cell broken each method and decolorization processing each method, the generalized analysis, the choice ultrasonic wave cell break method the way which unifies with the proteinase water solution carries on the decolorization to the goose blood protein the craft. In comparison, these two methods combination has the hematolysis highly effective thorough, the protein rate high, the decolorization effect good, the cost low, the side reaction are few, remains, the easy industrialization large scale production without chemistry, the product favor characteristic and so on absorption.The main research conclusion is as follows:1. The decolorization processing technical process is: Freshly prevents clotting the goose blood→centrifuge→precipitate→cell breaking hematolytic→enzyme hydrolysis→to adjust the pH precipitate heme→centrifuge→supernatant liquor→activated charcoal adsorption to decolorize→centrifuge→hydrolytic protein.2. Has determined the goose blood's blood corpuscle ultrasonic wave broken hematolysis craft: In the temperature 50℃under, compares the centrifugal fresh goose blood corpuscle after the solvent V_{red blood cell}∶V_{physiological saline} =5∶1 joins the physiological saline to process 15min under ultrasonic wave power 250W.3. Goose blood protein enzymolysis condition is: After the hematolysis had ended, will break after to dissolve in the blood to join the deionized water, the adjustment substrate concentration will be 40%, in the temperature 55℃under, adjustment solution pH 8.0, will join the papayin to carry on the enzymolysis, the enzyme compared to the substrate concentration [ E ]/[ S ] =8000u/g, enzymolysis 3h, in the reaction process through adds by drops 5mol/L NaOH and the HC1 solution maintains the pH to be constant.4. Decolorization crafts determination that: After the enzymolysis had ended, uses 5mol/L HC1 immediately the solution adjustment hydrolisis fluid pH4.5, the suppression enzyme activity termination reaction, in 55℃under settles the 50min precipitation heme. Then in 7000rpm centrifugal 10min, abandons precipitates, supernatant liquor in the temperature 54℃, under pH4.0, will join 2.5% activated charcoal to settle the decolorization to adsorb 65min. After the decolorization had finished centrifugal 10min precipitates under 7000rpm except the lower level activated charcoal, takes the upper formation protein hydrolisis fluid, for finally obtained hydrolytic protein.