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Study On Preparation And Utilization Of Protein Hydrolysate From Fish Waste Of Hairtail

Posted on:2009-07-15Degree:MasterType:Thesis
Country:ChinaCandidate:X M ZhangFull Text:PDF
GTID:2121360242997283Subject:Food Science
Abstract/Summary:PDF Full Text Request
The paper studied on preparation of protein hydrolysate from fish waste of hairtail, deodrization and utilization of hydrolysate which will provide reasonable and scientific basis.The results showed as follows:1.The content of total protein from fish waste of hairtail was 16.44%,fat was 17.83%,moisture was 75.50%,ash was 13.85%.2.Single Enzyme hydrolytic experimentsIn the experiment,papain,pepsin, flavor enzyme, Neutral Proteinase, trypsin were used to hydrolyze fish waste of hairtail.The effect of temperature,time,enzyme amount,pH value and solid/liquid ratio on hydrolysis were studied through the method combining orthogonal.Through the analysis of experimental data,the optimal proteases were determined.They were papain and trypsin.3.Compound enzyme hydrolytic experimentsIn the experiment,The best compound enzyme were used to hydrolyze fish waste from hairtail, The effect of temperature, the amount of enzyme,pH value and time on hydrolysis were studied through the method combining orthogonal.Through the analysis of experimental data,the optimal condition were determined. The temperature 55℃,time 6h,the amount of enzyme 4%,pH value 7.5.It showed that the temperature and the amount of enzyme was remarkable factor in the test through the variance analysis.4.Acid hydrolysisThe experiment of acid hydrolysis was studied in order to compare the effect of hydrolysis method on hydrolysis impact.The effect of temperature ,the concentration of acid,time and acid amount on hydrolysis were studied through the method of orthogonal trials.Through the analysis of experimental data,the optimum condition of acid hydrolysis was determined,the temperature 95℃,the concentration of HCl 6mol/L,time 6h,HCl solution/substrate ratio 6:1.The degree of hydrolysis at this condition is very high. the condition of acid hydrolysis was rigor, But the nutrient content of product was nearly destroyed,and the colour of hydrolysate was chocolate brown. So the main purpose of this paper is to compare with enzymatic hydrolysis. 5.Degreasing treatmentIsopropanol,hexane,Ethyl Acetate were used as extractant to degrease and compare with these degreasing effect respectively. The optimum degreasing reagent was Ethyl Acetate.6.Deodorizing treatmentThis research was mainly to study on fishy smell of protein hydrolysate of fish waste from hairtail by sensory evaluation, respectively using theβ-cyclodextrin,Gelatin,Starch and Activated Carbon in order to dedorizethis smell,investigated the protein loss rate and fishy smell and the optimum was Activated Carbon,adopted single factor and orthogonal experiment method,the temperature 65℃,time 40min,the content of Activated Carbon was 0.3%. Under this condition,not only reduce the protein loss rate,but also make the hydrolysate became light.It showed that the Activated Carbon can make the colour of hydrolysate became clear and can be debitterized and decolored.The quanlity of protein hydrolysate was improved.7.The paper studied on the emulsifying property,foamability and foam stability from protein hydrolysate of fish waste from hairtail which will provide proper research basis.And the antioxidation capacity of hydrolysate used in grease system was preliminarily studied.It showed that compound enzyme of hydrolysate used in grease system exhibited antioxidative activity.8.The proportion of lactic acid bacteria Streptococcus thermophilus:Lactobacillus bulgaricus= 1:1,seeding amount 2%,sugar concentration 1%,fermented for 36h unnder 42℃,the flavor of the fermentation liquid was the best.The fishy smell were removed.
Keywords/Search Tags:Hairtail fish waste, Protein hydrolysate, Oxidation Resistance
PDF Full Text Request
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