Studies On The Highly Specific MIPs' Design, Rationalized Self-assembly And Their Molecular Recognition

Molecularly imprinting, an important methodology for the straightforward preparation of antibody-like polymers, is being widely used in separation, extraction, catalysis and sensors. The dissertation presents detailed studies on MIP and their specific behavirors. The key endeavors were made on investigating the specific interaction and on how to improve the specificity of prepared materials. Using Boc-L-Phe-OH and related compounds as guiding templates, the self-assembly of template-monomers was studied with in-situ UV methods. The results indicate that the self-assembly can play a crucial role on preshaping the specificity of prepared polymers. Relative to the templates, too large or too small amount of monomers would not guarantee a specific framework matchable to the templates used. Only does the proper monomer-to-template ratio present a best specificity for molecular recognition. To acquire an imprint of a high fidelity, we have created a metallic pivot in the fabrication process of imprinting. The result indicates that the creation of metallic pivot causes obviously a higher specificity of recogition. Further studies show that this, in nature, can be the result of a larger stabilization on assembled complexes. Coordinate bond is much stronger than traditional hydrogen bond, which thus makes possible a larger restriction of monomers-, prepolymers-templates. Accordingly, the presence of metal mediator plays obviously a role on ameliorating the performance of prepared polymer. Finally, to realize the controllable imprinting recognizing process, combining a 'smart' technique into the preparation of catalyst, the influence of the operation temperature on the adsorption of the prepared polymer and its selectivity are discussed. At a relatively low temperature the prepared polymer, comparable to a traditionally imprinted one, presents a higher specific recognition for the template. It shows, however, no notable discrimination for the template and its enantiomer above the transition, due to the conformational change in the inner. The prepared polymer displays a 'switch' behavior, which thus presents a special mechanism for specific/nonspecific-commutating recognition.