Cloud Point Extraction And Ultraviolet-Visible Spectrophotometry In The Application Of The Use Of Copper Ions Detection

With the scientific and technological development, in geological, biological, and environmental sample analysis, the requirements of the regular g / L or mg / L level of trace elements, due to the sensitivity of analytical instruments and detection limit of the relationship between the number of machines in the determination of these trace elements in the sample will often encounter difficulties, and sometimes it is impossible, it is because, on one hand, the physical and chemical sample itself is not suitable for direct some state of, or analysis of very low Components of the sensitivity is not enough; On the other hand there is a matrix interference, or a lack of corresponding calibration standards and reagents. Therefore need the help of separation and enrichment technology to improve the analytical method sensitivity and selectivity.Cloud Point Extraction emerged in recent years is a new liquid - liquid extraction technology, it does not use volatile organic solvents, do not affect the environment. It neutral surfactant micelles aqueous solubility and cloud point-based phenomenon, triggered by changing the experimental parameters of phase separation, hydrophobic and hydrophilic substances material separation. Separation and enrichment as a means of extraction efficiency because of its high enrichment factor, simple operation, security, economic, easy to realize the advantages of the use by the people's grave concern and attention, and biological macromolecules Purification, small organic molecules and the separation of metal ions, such as the separation and enrichment receive a wide range of applications.Cloud Point extraction technology has been used for the separation and enrichment of trace elements and morphology analysis, and it can use a variety of detection technology. Spectrophotometric analysis with high accuracy and wide measurement, analysis speed, the advantages of less sample, and therefore has always been a cloud point of extraction technology with one of the main means of detection, the use of this technology is also known as Phase Separation spectrophotometry.The purpose of this paper is to cloud point extraction technology and UV - visible spectrophotometry combined to establish the actual samples of trace amounts of copper ion of the new method. Main research can be summarized as follows: (1)UV spectroscopy technology as a means of detection, copper reagents for the complexing agent, systematically affect copper ion cloud point extraction efficiency of the various factors to determine the optimal conditions for the extraction, and applied to environmental trace amounts of copper in water samples ion separation and determination;(2) UV spectroscopy technology as a means of detecting, amino acid as complexing agent, the effects of metal ions on the cloud point of copper extraction efficiency of the various factors study to determine the optimum conditions for the extraction, water and applied to the environment of Trace Separation and determination of copper.