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Study On Treatment And Resource Utilization Of Piggery Wastewater Using Biologic Humic Acids

Posted on:2009-07-04Degree:MasterType:Thesis
Country:ChinaCandidate:W J HuoFull Text:PDF
GTID:2121360245465034Subject:Microbiology
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The piggery wastewater has the characteristics of high ammonia, organism and suspending substances. How to treat them effectively and realize resource utilization have become important issues. The experimental base haogpen (amount of livestock:1200) used QS (a kind of biologic humic acids productions) to ferment piggery wastewater and obtained a great of economic benefits through"pig -fermention - fish - plant"ecological breeding model. According to the application effects of QS, this paper consists of the following three areas of study: 1. investigating the fermentation effects of QS on piggery wastewater 2. monitering the water quality of"pig-fermention-fish-plant"model and analyzing the effect of processing mode 3.investigating the types and quantities of microbes in QS and identifying of functional microbial to further explore the role of QS. The results are as follows:1. In the experiment, there are four treatments .Afeter 8-day fermention, the CODCr removal values were 39.21%(Ⅰ), 42.05%(II), 33.09%(III), 33.05%(Ⅳ); NH4+-N concentrations were by an increase of 16.5%(Ⅰ), 14.5%(II), 13.7%(III), 14.6%(Ⅳ); total phosphorus(TP) values had no significant changes, but PO43- values were on upward trend, with the increase percentage of PO43-/TP values followed by 25.6%(Ⅰ), 31.3%(II), 30.8%(III), 27.7%(Ⅳ); All the quantity of culturable microorganism decreased from 107~108 cfu / mL to 105 cfu / mL and all the quantities of fecal coliform bacteria decreased from 108~109 MPN / L to 106 MPN / L.According to statistical analysis, the CODCr removal percentages of treatmentⅠandIIwere significantly higher than those of treatment III andⅣ, and other parameters of treatments showed no significant differences(α=0.05). However, it could be found that the CODCr removal percentages and PO43-/TP value increase percentages of treatment II were higher than other treatments in three batches of tests.2 Applying QS to piggery wastewater fermentation in field for 8 days, the changes of parameters were as follows: CODCr removal percentage, 70%~75%; NH4+-N increase percentage,18%;TP removal percentage,28 %; PO43-increase percentage ,35.5 %; the quantity of culturable microorganism ,105 cfu / mL,and the quantity of fecal coliform bacteria decreased from 107 MPN / L to 1.67×10~5 MPN / L.The organic matter in wastewater could be degraded effectively by fermentation,and the increase of available nitrogen and phosphorus was also beneficial to fishing.3.The monitoring results of the water quality of"pig-microbial-fish"pound were as follows: the pH value , 7.2~8.1; the DO value , 4.6~6.6 mg/L; the concentrations of NO2--N, NH4+-N, TP, CODCr were 0.1~0.3mg/L, 0.9~1.3mg/L, 0.6~0.9mg/Land 70~90 mg/L respectively;the quantities of culturable microorganism and fecal coliform bacteria were 104 cfu / mL and 10000~25000 MPN / L.The monitoring results of the water quality of"pig- fish"pound were as follows: the concentrations of NH4+-N, TP,CODCr were 2.5~3.0mg/L,3.0~3.7mg/L and 130~150mg/L respectively,and the quantity of fecal coliform bacteria was 105 MPN / L.The monitoring results of oxidation plant ditch were as follows: the concentrations of CODCr, NH4+-N, NO2--N, TP were 10~40mg/L,0.9~1.3mg/L, 0.02~0.2mg/L and 0.14~0.2mg/L respectively. The quantities of culturable microorganism and fecal coliform bacteria were 103~104 cfu / mL and 103~104 MPN / L.The monitoring results of"pig-microbial-fish"pound water meant that the parameter values were all in safe. All the datas of"pig- fish"pound water were much higher than those of"pig-microbial-fish"pound water and under fishing water safety standard.There were potential dangers of production existed in"pig- fish"pound. The ditch water was the best among the three pounds, and the oxidation ditch might play some ecological function to improve fish pound water quality. The"pig-microbial-fish-plant"ecological breeding model could guarantee the production safe basically.4. QS contained culturable microorganisms with a total of 108 cfu / g and ten kinds of bacteria were released from the repeated strains, which had the ability of nitration, Chlorpyrifos (1%) degradation, Quintozene (0.1%) degradation, inorganic phosphorus solution and resistance against fecal coliform bacteria. They belonged to Bacillus badius, Bacillus cereus, Bacillus Licheniformis, Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Paenibacillus.
Keywords/Search Tags:biologic humic acids, piggery wastewater, resource utilization, microorganism
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