| The contents of heavy-mental in soil are far more beneficial to human being, which have been being harmful to the quantities and quality of agricultural products. How to decrease the contents of heavy-mental in soil has become a focus problem which has attracted most researchers on soil science. With the development of biological science, the remediation for soil pollution using biotechnologies will be one of the most available measures. As a major component of soil pollutants, Arsenic pollution has become a hot spot of soil-pollution-related researches.Using arsenic(As) hyperaccumulator Pteris vittata L. as experimental plant, the pot experiment was layout in greenhouse by combining different cultivation ways,fertilization concentrations and management measures, through which aimed for finding out the optimal cultivation condition to enhance Arsenic- absorbing ability and increase the bioremediation efficiency of Arsenic-contaminated soil. Meanwhile, the maize with high biomass was used for another trial plant, by adding inocula, earthworms and straw to trial field, to investigate the remediation efficiency of Arsenic-contaminated soil by measuring the contents of Arsenic and Mercury in soil.The effects on spore propagation speed of Chinese brake were investigated by pot experiments in different treatments including physical, chemical and inocula aspects. The experimental data shows that physical treatments (mechanical friction and low temperature) and chemical treatments (GA3 and 6-BA soakaged) accelerated germination speed and shorten germination time of the Chinese brake spore. Compared with control, the longest time was spent on the treatment by adding inocula to the soil but the shortest time was used from germination to round-leaf formation. The height of petiole reached the maximum value by adding inocula after 140 days from sowing, so adding inocula is beneficial to grow quickly of Chinese brake seedling.The effects of different cultivation ways,fertilization concentrations and management measures on Pteris vittata L. growth were characterized after 5-7 pinnate leaves appeared. The experimental data show that adding proper content of N,P,K fertilization promote growth of Pteris vittata L. seedling, based on which some more inocula added to soil got a better result. Appropriate component proportion not only promote seedling growing of Pteris vittata L. but also increase Arsenic accumulation. Our research also focused on the changes on the absorbability of As and Hg in crop and soil, especially the forms change of chemical element, As and Hg, and enzyme activity in soil under biotic interaction around the rhizosphere soil under the microbe-soil animal-plant interaction soil environment contaminated by As. Our data show that adding inocula, earthworms and straw to trial field separately all accelerated maize grow and had higher biomass than control. Underground part of the maize accumulated more As than aerial part, which is beneficial for remediation of As-contaminated soil, and EMR has the highest As contents among all treatment. There are no significant differences of As contents among all treatments on crop aerial part. The As contents under EMR treatment decreased from root, leaf, stalk to panicle in maize, which demonstrated that EMR not only helpful to biomass increasing, but also beneficial for As absorbability of underground part. acid phosphatase activity is also increased under this treatment, all of which characters are beneficial for biosafety.
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