Investigation On Isolation, Structural Analysis And Activities Of Water-Soluble Ploysaccharides From Mussel Boiling Juice

Mussel boiling Juice (MJ) was concentrated from decoction of fresh mussel which was boiled for 24 hours, and contained many nutritional and functional components like proteins, saccharide and so on. In this study, the water-soluble polysaccharides (MJPs) from MJ were isolated, and then their physical and chemical properties, structures and activities were investigated to help to make full use of the resources, to develop new functional foods and to improve the economic benefits.The MJPs was extracted by alcohol fractional precipitation, free proteins and colors were removed by Sevage with papain method and charcoal, respectively. The homogeneity of MJPsâ… and MJPsâ…¡which were isolated and purified by Sepharose 4B column and dialysis membrane was determined by UV absorption spectrum, aceto-cellulose-thin layer-electrophoresis and HPGPC. And then their primary structures were analyzed by GC, IR and NMR. Furthermore, the anti-bacteria activity, blocking synthesis of NDMA and anti-oxidation activity of MJ and MJPs were measured by anti-bacteria cup method, standard curve method and DPPH·method. Lastly, the immunological activity, anti-tumor activity and inhibitory effect on melanin of MJPsâ… and MJPsâ…¡were tested.The results showed that saccharide in MJ were nonreducing sugar mostly, and purified MJPsâ… and MJPsâ…¡were tested to be homogenous. The structural analysis indicated that MJPsâ… , with a molecular weight of over 2.0×10⁶Da, was composed of glucose as its major components. Its structure was speculated as follows.MJPsâ…¡, with a molecular weight of over 2.0×10 Da, was also composed of glucose as its major components. Its structure was speculated as follows.{→2)-α-D-Glcp-(1→}n.The MJ and MJPs did not display significant inhibitory effects on Staphylococcus aureus, Saccharomyces carlsbergengensis Hansen, Escherichia coli Castellani et Chalmers and Vibrio parahaemolyticus. In the experiment of scavenging NaNO₂ and blocking synthesis of NDMA, MJ showed strong activities, while MJPs only showed low activities; In the experiment of scavenging of DPPH·, MJ's IC₅₀=3.80μg/mL measured by soluble solid, MJPs'IC₅₀=1.12μg/mL, while the TBHQ's IC₅₀=7.55μg/mL. For MJPs, the strongest Inhibitory Rate was 59.9%, which was lower than TBHQ, of which the strongest Inhibitory Rate was 89.9%. However, the IC₅₀ of MJPs was much lower than TBHQ. MJPsâ… showed significant positive adjusting effect on T cells and B cells. MJPsâ…¡exhibited significant negative adjusting effect on B cells in low concentration and adjusting effect on T cells in high concentration. Both of them showed the inhibitory effects on EBV activation and inhibitory effects on melanin, with low toxicity on Raji and melanoma cells.MJPs displayed strong inhibition of DPPH·in low concentration. MJPsâ… and MJPsâ…¡displayed immunological activities, anti-tumor activities and inhibitory effects on melanin with low toxicity on Raji and melanoma cells, which indicated that the Mussel Juice Polysaccharides may be valuable on developing the white-makeup ingredients and functional foods and inducing the cytotoxicity when added in other strong anti-cancer agents with high cytotoxicity.