Jellyfish Collagen Extraction And Ultrafiltration Application In Collagen Purification

Collagen, an important family of structural protein, has been widely applied in the field of medical treatment, health care and hairdressing. Jellyfish are abundant in collagen, as well as fat content is less than 0.01%. Jellyfish collagen has the effect such as antisepticise, treatment of arthritides, health care, which is hopeful to become functional food or healthcare products. Therefore, utilizing abundant jellyfish resources and recycling useful waste during jellyfish process in order to upgrade their utilization. It has the significant directive meaning to widen collagen extraction resources and application.In this paper, two methods named as acid extraction and pepsin extraction were used to extract collagen from Jellyfish. Then, utilize ultrafiltration to isolation and purification instead of traditional technology such as dialysis and salting out. In the discussion of optimization of extraction conditions, the results showed the optimal condition of acid extraction was using 0.5mol/L acetate acid, ratio of material to liquid 1:3.0, extraction time 96 hours, and that of pepsin extraction was using 0.5mol/L acetate acid extract 96 hours, with a ratio of material to liquid 1:4.0, then centrifugation, the non-soluble go on dissolving in 0.5mol/L acetate acid in 96 hours, add in 4.0 % pepsin, ratio of material to liquid 1:4.0.In addition, more attention was payed on the variation of collagen extraction rate by different substrate concentration. Based on Michaelis-Menten equation, a dynamical model was developed to simulate the association of substrate concentration with reaction rate. The mathematic model can be expressed asFurthermore, effects of operating time and pressure on collagen ultrafiltration have been highly investigated. In addition, the influences of different sorts of acid solution, including in 0.5%collagen-H₂O solution, 0.5%collagen-0.5mol/L acetate acid, 0.5%collagen-0.01mol/L HC1 and 0.5%collagen-0.5mol/L citrica acid, were researched. In conclusion, different acid solution demonstrated different ultrafiltration results. Moreover, a mathematic model was developed to simulate the relationships of membrane flux with pressure, time and collagen concentration. The mathematic model can be expressed as All of these supplied a profitably foundation for jellyfish collagen commercial production.