Research On The Extract And Preparation Process Of Broccoli Seed

The broccoli seed is dry and mature fruit of broccoli from crucifers plant. Mainly contain isothiocyanate as the alive compositions. Epidemiological and laboratory studies suggest that dietary broccoli may prevent or delay a variety of cancers. Broccoli and other crucifers contain a relatively unique family of secondary metabolites called glucosinolates. Glucoraphanin, the major glucosinolate in broccoli, is hydrolyzed by an endogenous plant myrosinase to form isothiocyanate. There have been health care products on sale in abroad now, but the research in our country is just to start and the area of health care products is still blank.So this experiment through the broccoli extract a comprehensive study, research and development into a new and anti-cancer effectiveness of the health-care products "broccoli seed extract tablets."Using orthogonal design approach to the yield of sulforaphane indicators for the broccoli seeds hydrolysis conditions for screening to determine the best broccoli seed extract concentration process: The crushed seeds of broccoli, over 40 mesh screen , then by adding 30 times the volume of pure water in a water bath at 35℃1 h after hydrolysis then adding 1 times the amount of ethanol enzyme inactivation and mustard, centrifugal, the residue was washed with a small amount of water fully after centrifugation. Mergering the supernatant, condensed with rotary Evaporation-enrichment (per 100 g seed condensed to 100 ml), then broccoli seeds concentrated liquid are obtained.According the physical and chemical properties of sulforaphane and combination the the data of the stability, the eluate was selected, sulforaphanusing was seprated using silica gel column after three times .The purity of sulforaphane monomer was detected by high performance liquid chromatography ,the result is 95.29%.According to the physical and chemical properties of raw materials preparation, to combine the characteristics of a type of tablet, accessories through screening, the process of preparing broccoli extract tablet is: first microcrystalline cellulose, starch, CMC-Na are mixed according to the ratio (20:20:1 ); citric acid dissolved in concentrated liquid in the broccoli, regulating pH value of 3.5.Then added to the concentrated liquid blending of accessories, uniform mixing, system by adding 30% ethanol soft wood, 16 extrusion head off screen granulation, 40℃oven drying 2 h, Whole sieved out, by adding 1% magnesium stearate blender, pressed, every tablet is 0.5g, the thickness is 2.1mm-2.2mm. Pressed powder to screen films, in white opady film-coated pot packets clothing, that is a. For the preparation of quality standards, used mainly by high performance liquid chromatography in the preparation of the main active ingredient sulforaphane were determined, in each tablet of broccoli extract the content of sulforaphane is not less than 1 mg / tablet.