The Polysaccharide-extraction And Comprehensive Utilization Of Laminaria Japonica

There are many active components in laminaria japonica: such as fucoidan, sodium alginaf, mannito, dietary fiber. Fucoidan has been the focus in recent years. The poupose of the paper is to find the best conditions of extracting all the active components in laminaria japonica. Through single factor and orthogonal test, we got the results:Water extracting, acid extracting and ultrasonic wave extracting are three different methods to extract fucoidan from laminaria japonica. The best conditions of water extracting was: time 11h, 30ml/g water, temperature 90℃; The best conditions of acid extracting was: time 4h, 10ml/g water, pH 2; The best conditions of ultrasonic wave extracting was: the amount of laminaria japonica 2g , time 45min. Water extracting method has the highest product yield and the least impurity. So we can say that the water extracting method is the best method. After extracting, the extracting liquid was concentrated to 1/2, then add ethanol until its concentration was 60%, we can get the most amount of fucoidan.After water extracting, the fucoidan was purifed by CaCl2 method. The product we got had more fucoidan and more algin compared to the product extracted from acid extracting and purifed by ethabol grading method. We compared the antioxidant nature of the two products above though DPPH. We found that although the first one had more impurity, it had the higher antioxidant nature. So next , the first one was separated by IC (type: DEAE-cellulose52) , we got three waves: F1,F2 and F3. Then we used GPC (type:SephadexG-100) to got their molecular weights.The best condition of extracting mannito was: temperature 90℃, time 4h , 30ml/g water. The laminaria japonica after extracting fucoidan was dried and treated by enzyme to get dietary fiber. The best condition was: cellulose enzyme 100U/g, protein enzyme 2000U/g, time 1h. The best condition of extracting dietary fiber which can dissolve in alkali was: time 2h, the concentration of NaCO3 1%, temperature 60℃, 30ml/g Na₂CO₃. The dietary fiber was applied in noddle, by adding 50g dietary fiber to 1kg flour, the noodle was the best.