Study On BSA-Imprinted P(AM-NVP) Beads

At present much remains to be done in order to clarify the mechanism of selectivity of the protein-imprinted polymer and to find the optimal experimental conditions. In this thesis, protein-imprinted P(AM-co-NVP) hydrogel beads were prepared, the adsorption and recognition properties of the beads were studied, as well as the imprinting mechanism was discussed.Protein-imprinted P(AM-co-NVP) beads were prepared via inverse-phase suspension copolymerization, using acrylamide and vinylpyrrolidone as monomers and N,N'-methylene bisacrylamide as crosslinker. The results suggested that the reaction temperature, the ratio of oil to water, the amount of ethyl cellulose as dispersant, initial monomers composition, the drop manner and the amount of initiator, the stirring speed, the pH of the buffer and the elution process have great influence on the morphology such as diameter of the hydrogel beads. The adsorption capacity of BSA-imprinted beads was three times as much as that of non-imprinted beads. The selectivity test of imprinted beads showed that imprinted gel beads exhibited good recognition for BSA. The effects of the copolymer composition, the ionic strength and pH of the buffer value on the adsorption capacity and imprinting efficiency of the beads were also researched. The mechanism was explained that the copolymerization composition have great influence on the swelling ratio, surface special swelling and adsorption capacity of the protein-imprinted P(AM-co-NVP) hydrogel beads. The principle of condition similarity was proposed that the protein imprinting and recognition process need to be accomplished in the similar environment.