The Isolation And Chemical Structure Reaserch On The Polysaccharides In Laminaria Japonica

The extraction, purification technology, refined polysaccharide classi-fication of Laminaria Japonica (LJ) have been studied. Two of Laminaria Japonica polysaccharide (LJP) molecular weight was identified and structure of LJ was preliminary studied.The technics condition of the sulfate-phenol method to mensurate polysaccharide was determined. The result of experiment indicated that there were polysaccharide solution of 1 ml, phenol solution of 1.2 ml, concentrated sulfuric acid of 6mL, 10 ml of distilled water to supplement, natural cooling, scanning in the 487 nm. The optimal conditions were obtained on the basis of studying orthogonal experiment. The result of experiment indicated that pH at 2 and extracting temperature at 65℃, leaching rate of LJP was up to the maximum by extracting 3h at 40:1.Leaching rate of LJP was fixed after extracting 2 times and leaching rate of LJP was 8.094%. Meanwhile the leaching trend of polysaccharide was explained with extraction theory.The sevag method, TCA method and papain method were studied and the optimal conditions were obtained in deproteinization from LJP. The result of experiment indicated that: ratio of enzyme to base material 1.2%, pH 5.0, the eliminating rate of protein was up to 66.67 % by reacting 1.5h at 70℃. The leaching rate of polysaccharide increased to 13.17 % by adding papain to extraction process, and the eliminating rate of protein was up to 65.5 %.Activated carbon, DA201 resin, DA202 resin, DA203 resin four different columniation was researched in the process of decolorization. The results showed that DA202 resin is one of resin with the smallest amount, the highest pigment removal and the lowest rate of losing polysaccharide,Three components L1, L2 and L3 were obtained through DEAEC-52 celluloseion exchange column. The contents of L2 and L3 were higher, and were the major components of LJP. L2 and L3 were analysized by DEAE-SephadexA-75 gel column chromatography, UV detection, freeze-thaw analysis. L2 and L3 components were proved to be homogeneous components. By DEAE-SephadexA-200 gel column chromatography, the molecular weight of L2 and L3 were 131.8 kD and 93.3kD respectively. By Infrared spectrometry, L2 containedα,βglycosidic bond, and was a kind of acidic sulfate polysaccharide. L3 was a high viscosity acidic polysaccharides.