Research On Bioremediation Experiment Of Oily Soil In Daqing

As we know, oil is precious nature resource. It can be able to provide power for national economic, but if it came into environment, the oil may become contamination. Some scholars found that a number of compounds had potential of causing abnormal, causing cancer and causing mutation. These compounds included chain alkanes, cycloalkanes, aromatic hydrocarbon and mixed atom compounds. Especially, oil was brought into soil, it would not only destroy environment, but also made human health in danger by food chain. Therefore, the research was carried out on bioremediation experiment of oily soil in Daqing, which had important practical significance.The plants that familiar in Daqing area are taken to study the phytoremediation of oily soil through seeing about on the spot in this paper. The method of bioslurry was adopted for microbial remediation, and every factor was analyzed about the influence degree to microorganism after twenty four days shaking. As for plant-microorganism combined bioremediation experiment, we made sampling on locale but measured in a laboratory to research ground oil that placed outdoor in different time.1. Phytoremediate oily soil in condition of indoor environmentThe plants such as cattail, phragmite and leymus chinensis and so on were growed in different concentration oily soil. We would collect the soil every other time for analyzing the content of petroleum contamination and the degradation circumstances of every compound in oil by gas chromatography. The results indicated that the plants had degradation effect to petroleum in soil. Cattail had more degradation ability relatively. The degradation speed to every compound of petroleum was different from each other, in other words, the speed of alkanes that have low molecular weight was obviously quickly than ones have high molecular weight. With the increasing of growth days, the content of petroleum organic matter reduced gradually.2. Simulative experiment study of microbial remediation in laboratoryIn this experiment, oily soil needs to be confected by handwork. 15g soil sample was weighted and 150ml water was measured, mixed them up and then turned into slurry whose concentration was 9.09%. These samples were put in an oscillator of air bath to vibrate, and were analyzed the influence of vary factors about microbial remediation. The results indicated that temperature had a great effect to microbial remediation efficiency, optimal temperature was 40℃. The degradation ability of microbe had no direct relativity with nutrition matter, and the infection of surfactant was extremely tiny. The experiments concluded that the best experimental conditions were: the proportion of nutrition (N/P) was ten to one, electron accepter (H2O2) was cumulated to 14ml, containing water 50%. Petroleum contamination concentration was an important factor, though petroleum of low concentration had not great significance, petroleum of high concentration had a certain extent poison function for microbe. 3. Experimental research and analyze on integrative degradation effect of oily soilOily soil samples that buried in different places on 2004 year and September month were gathered, and then the samples were sent back to laboratory for analyzing. The investigation suggested that there were microbes indulged in petroleum, and the content of organic matter lessen evident than last year. The places in which had plants grew were suitable to exist for rhizosphere microbes, and the activity of these microbes were strengthened, so degradation effect was better than the places where had no plants. From the quantitative analysis of gas chromatography, we know that the degradation speed of n-alkane bigger than iso-paraffins.