Studies On The Submerged Fermentation Of Auricularia Auricula By Adding A Certain Kind Of Chinese Traditional Herb Medicine

Auricularia Auricula is a kind of fungi which is both edible and medicinal.The extro-polysaccharide of Auricularia Auricula has various biological activities,such as anticogulation,antilipidemic,antiplatelet,antitumor,etc.In this paper,the primary purpose of the research was to produce better and more extro-polysaccharides by submerged fermentation from Auricularia Auricula.After examined six kinds of Chinese traditional herb medicines from Guizhou province,shaking flask fermentation, extraction,and the techniques in 15L fermentor were well studied in this paper.The effects of water and ethanol extracts of six kinds of Chinese traditional herb medicines,which were from Guizhou province,on the submerged culture of Auricularia Auricula were firstly studied.The results showed that the yields of extro-polysaccharide were increased in different levels when added any water extracts of the six medicines. But the results varied when added ethanol extracts.Take both biomass and extro-polysaccharide into consideration,we chose the ethanol extract of Gastrodia elata.The effects of some nutritional factors on the submerged fermentation of Auricularia Auricula were studied.And the results showed that glucose,yeast extract, salt and ethanol extract of Gastrodia elata in suitable levels could promote the yield extro-polysaccharide.An optimum medium was obtained by responding surface method experiment.The medium composition was as follows:glucose37.81 g/L,yeast extract 10.23g/L,ethanol extract of Gastrodia elata 30.00g/L,KH₂PO₄3.94g/L,MgSO₄1.97g/L.The maximum extro-polysaccharide yield reached 2.65g/L when used the medium above.The optimum fermentation conditions were as follows:initial pH5.66(natural), culture temperature28℃,inoculation age 4-5d,inoculation quantity 10%,80 mL medium in 250mL shaking flask.The extraction techniques of Auricularia Auricula extro-polysaccharide had been studied as well.The results showed that after the concentration of ethanol reached 30%in the first step,the concentration of ethanol should reach 75%in the second step. And then,deposit at temperature 4℃for 24h.The tests in 15L fermentor showed that higher ventilatory volume was good for the submerged fermentation of Auricularia Auricula.And considering the practice, 6v/v/m was better.The result of temperature controlled strategy study showed that form 0 to 84h,it should be cultured under 28℃,and form 84 to 144h it should be cultured under 25℃.The result of pH controlled strategy showed that form 0 to 84h, it should be cultured under pH5.1,and form 84 to 144h it should be cultured under pH 5.7.Under the optimum conditions,the yield of extro-polysaccharide reached 3.42g/L.