The Synthesis Of Nutrient Fortificant-amino Acid Chelates And Its Application

AAC(Amino acid chelate) is a new type of microelement additive with good physic-chemical sTab.ility and bioavailability, its adverse effect on vitamins and UFA is very weak.We synthesized five AACs as follows: glycine chelated ferrous, glycine chelated zinc, glycine chelated calcium, methionine chelated copper and methionine chelated Manganese(â…¡).Firstly glycine chelated ferrous, glycine chelated zinc, glycine chelated calcium were synthesized. Then methionine chelated Manganese(â…¡) was synthesized with MnCl₂ and methionine. The optimum synthesis conditions were found as follows: molar ratio of ligand 2:1, 90 min, 80℃,and the yield of production was 89.91%. Methionine chelated copper was synthesized with CuSO4 and methionine, the optimum conditions were: molar ratio of ligand 2:1,add 3.25mL 30% NaOH to 50 mL 6% methionine solution,and the yield is over 90%.According to"7+1"nutritional program, Four kinds of flour fortificants were prepared with different mineral sources: inorganic, organic, chelate and mixed. The retention of Fe(â…¡), vitamine A , vitamine B1, vitamine B2, nicotinic acid and folate was studied during storage at ambient temperature and 45℃. The results indicate that oxidation sTab.ility of Fe(â…¡) in chelate group is better than inorganic group; Nicotinic acid has good sTab.ility and keep a high level in all groups; retention rate of vitamine B2 was about 80% after storage ,no big difference was found among the 4 groups; retention rate of the other four kinds of vitamines in chelate group is the highest, mixed and organic group follows, inorganic group is the lowest. Besides,we find the degradation kinetic equation for vitamine A , vitamine B1.We also studied the sTab.ility of Fe(â…¡) ,the retention of VA,VC,VE and POV during storage when adding inorganic salt, chelates we synthesized into infant formulae. The results indicate that the oxidation sTab.ility of Fe(â…¡) in chelate is better than FeSO4,the retention rate of VA,VC,VE keeps highest in chelate group infant formulae, followed by mixed group, and inorganic group is the lowest. higher temperature could accelerate the degradation of vitamins mentioned above. The POV of three group of infant formulas all become higher after storage: inorganic group >mixed group > chelate group, which suggests that AAC is better than inorganic salts when adding into infant formulae.