The Study Of β-Carotente Microemulsion Agentia

The density functional theory method at the B3LYP/6-31G* level were carried out for calculating the physiochemical parameters of the structural units of 17 familiar solvents, which were used to investigated the quantitative structure property relationship(QSPR)of the solubility ofβ-carotene.Microemulsion systems which were used to envelopβ-Carotente were prepared. A microemulsion containing Tween80, ethanol and ethyl butyrate was developed to envelopβ-Carotene with psedu-ternary phase diagram; The O/W, W/O and B.C. regions were divided with conductance method, the influences of temperature, salinity, pH as well as preservative on the microemulsion regions were also considered;The viscosity and the particle sizes variation in two systems of empty microemulsion and that containingβ-Carotene have been investigated; The effects of sunlight, temperature and pH on the stability ofβ-Carotene were comparatively studied both in microemulsion and in ethyl butyrate solution.Results of quantum chemistry calculation showed:The best fitted QSPR model obtained has good correlation, small standard deviation and can forecast the solubilities ofβ-carotene in unknown solvents simply and quickly;The solubilizing capacity ofβ-carotene in these solvents: THF > ethyl butyrate > Hexane > 2-propanol.Study of microemulsion showed that O/W microemulsion formed when water weight content was above 61.5% in the mixture; The microemulsion regions decreased slightly with the increase of temperature and the decrease of pH; Salinity and preservative had hardly influences on the microemulsion regions. Thatβ-carotene affected obviously the mesophases curvature and advanced the formation of B.C., and thus enlarged the bicontinuous and O/W regions; DLS showed that the droplets in microemulsion containingβ-Carotene, as well as, in empty microemulsion prepared after 3 days shrunk sharply, then kept remarkable stability even after a month; The figure showed that theβ-Carotene was much more consumed in the sunlight and high temperature, Which denoted that sunlight and high temperature had obviously influences on the stability ofβ-Carotene both in microemulsion and in ethyl butyrate solution; Theβ-Carotene contained in the microemulsion, compared with in ethyl butyrate solution, was much more stable. The test of influencing factors indicated that theβ-carotene microemulsion should be preserved at room temperature and avoided light.Some jobs were created in this work, envelopedβ-Carotene in microemulsions, madeβ-Carotene not to be easily oxidized and have good stability.1. The density functional theory method at the B3LYP/6-31G* level were carried out for calculating the physiochemical parameters of the structural units of 17 familiar solvents, which were used to investigated the quantitative structure propert relationship (QSPR) of the solubility ofβ-carotene. The best fitted QSPR model obtained has good correlation, small standard deviation and can forecast the solubilities ofβ-carotene in unknown solvents simply and quickly.2. The O/W microemulsion to envelopβ-Carotene was formed steadily, which changedβ-Carotene into water-soluble liquid in water and not being easily oxidized in atmosphere, light or temperature.3. The diameters ofβ-Carotene microemulsions were around 80nm, which was also believed to enhance its bioavailability.