Research Of Extraction And Antioxidant Activities Of Flavonoids From Hetian Jade Jujube

Jujube(Zyzyhpus Jujuba dates) is a Rhamnacede fruit of Zizyphus Jujuba Mill plant.It is a Chinese unique fruit and is well-known being abundant in nutriments,vitamin,minerals and bioactive substances such as cAMP etc.Plant flavonoids have many health care functions.They can be used as a treatment of antioxidant,anti-aging,cardiovascular illness,blood fats and so on. Through the processes of Hetian jade jujube flavonoid extraction,separation,qualitative and antioxidant activities research,the paper with Hetian jade jujube as a raw material is aimed at providing the technological parameter and the theory basis for the Hetian jade jujube's processing use.This has the important meaning to enrich scientific knowledge of the food industry and the promotion red jujube industrial production development.The main results are as follows:1.Microwave-assisted extraction for flavonoids from Hetian jade jujube is the optimum extraction method.The optimum conditions of microwave-assisted extraction from Hetian jade jujube are:the ethanol concentration:80%;the microwave power:420W;the extracting time:90 second;the solid-solvent ratio:1/24.In the above conditions,the extracting ratio of flavonoids from Hetian jade jujube:412.25mg/100g.The optimum parameter of other extraction method are as follows:The optimum conditions of ethanol extraction from Hetian jade jujube:the ethanol concentration:80%;the extracting temperature:70℃;the solid-solvent ratio:1/24 and the extracting time:4 hours.In the above conditions,the extracting ratio of flavonoids from Hetian jade jujube is 332.26mg/100g.The optimum conditions of ultrasonic extraction from Hetian jade jujube are:the ultrasonic power:200W; the extracting time:40 minutes and the solid-solvent ratio:1/24.In the above conditions,the extracting ratio of flavonoids from Hetian jade jujube is 378.25mg/100g.The optimum conditions of enzyme add extraction from Hetian jade jujube are:pH value:5.5;adding cellulose:4mg and pectase: 2mg;treating time:1 hour and treating temperature:45℃.In the above conditions,the extracting ratio of flavonoids from Hetian jade jujube is 382.53mg/100g.2.AB-8 macroporous resin has good performance of adsorption and desorption ability to flavonoids.The optimum conditions of separation and purification for flavonoids from Hetian jade jujube are as followings:the loading amount:100～120mL;pH value:5～6;the current velocity of sample solution:1mL/min;the flavonoid concentration in the liquid:0.2mg/mL;the eluting reagent: 70%ethanol;the eluting velocity:1mL/min and the eluting reagent volume:5VB.In the above conditions,the purity of flavonoids from Hetian jade jujube is 28.5%.3.Flavonoids from Hetian jade jujube have antioxidant activities.The eliminating free radicals activity to the three kinds of free radicals and total reducing power would be enhanced along with the concentration of flavonoids from Hetian jade jujube.In hydroxyl radical(·OH) system,the scavenging rate of·OH by coarse flavonoid and pure flavonoid could approach to 78%and 83% when the experimental concentration was 1mg/mL.In superoixide anion(O₂^-·) system,the scavenging rate of O₂^-·by coarse flavonoid and pure flavonoid could approach to 44%and 66% when the experimental concentration was 1mg/mL.In the 1,12-diphenyi-2-picrylhydrazyl radical (DPPH·) system,the scavenging rate of DPPH.by coarse flavonoid and pure flavonoid could approach to 74%and 78%when the experimental concentration was 1mg/mL.In the same concentration,the scavenging rate by pure flavonoid is bigger than coarse flavonoid.The total reducing power of coarse flavonoid and pure flavonoid were almost the same when the experimental concentration was under 0.4mg/mL,but the total reducing power of pure flavonoid is much better than coarse flavonoid when the experimental concentration was onto 0.4mg/mL.4.The optimum conditions of oral liquid of flavonoids from Hetian jade jujube which were determined by 0.618 method and uniform interaction design obtained was as follows:(each 1L oral liquid) pure flavonoids from Hetian jade jujube:10g,zinc acetate 33.45mg,honey:106.4g, sucrose:39.14g,citric acid 1.000g,carrot juice:1.35%,jujube juice 17.35%.The oral liquid assumed primrose yellow,slightly had the caramel to be fragrant,sour and sweet palatable,smooth,had no settling and obvious impurity.