The Exploratory Experiment Of Click Chemistry In Vivo Marking Application

Click chemistry is a sort of high-efficient modular and specific reactions which is represented by Cu-catalyzed Husgen 1,3-dipolar cycloaadition. With click chemistry, we can get two different molecular (include large bio-moleculars and special groups) linked together quickly and effectively. Thus, click chemistry has drawn the attention of researchers in pharmaceutical chemistry and bio-chemistry field recent years. This dissertation is a exploratory wok on the application of click chemistry in vivo marking, and the biological endurance problem of the relevant catalysts.4-ethynyl-N-ethyl-1,8-naphthalimide is a non-fluorescent alkynyl donor which can appear strongly fluorescent after it react with azides with the presence of CuI catalyst. Use this phenomenon as a method to judge whether click chemistry reaction has been beginning , we study the possibility of using click chemistry to mark a cancer cell. Then focus on the toxicity of Cu catalyst, which is the obstacle of the vivo application of CuI–catalyzed click chemistry, proposed the possibability that galactose oxidase(GAO), a natural Cu-protein, can take the place of the ordinary CuI catalyst. Then change the Cu catalyst of click chemistry reaction system with the GOA which are treated in different ways by turns, compare their efficency by observing the apparent fluorescent during one week's reaction. All the click chemistry reagents were synthesized in laboratory, and here we have some description of some phenomenon which is worth to caring and describe some improvement of relevant synthesis operation.