| According to the character of chloramphenicol palmitate and liposome, a systematical study was carried out for the preparation of chloramphenicol palmitate liposome, which will be more efficient and less toxicity. The following works have been done:RP-HPLC method was developed for determination of drug concentration. The method offered a linearity of 28～52μg·mL-1 for chloramphenicol palmitate(r=0.9999), the average recovery rates is 100.9%. The liposome is separated by exclusion chromatography with Sephadex LH-20, and the result is tested by RP-HPLC.The encapsulations of different preparation methods were compared. The TFV was selected to prepare liposome. The highest encapsulation of 92.1%±0.40 was gained. Orthogonal text was used to grope the optimal technical condition. The appearance, pH, size, encapsulation of liposome and residual quantity of solvent were observed.The liquid liposome is suspensible, easy to polymerize and cause the medicine leakage. In order to grope the optimal technical condition of the rehydrate liposome, orthogonal text was used. We conducted the research to the stability of the rehydrate liposome, and find out the conserved condition at 4℃, lightless is good for the liposome according to 3 months observation.
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