Isolation And Identification Of Tritepene Saponins And Active Polysaccharides From Polygonatum Sibiricum Red

Polygonatum sibiricum Red., which is distributed extensively in China, has a great prospect. In this paper, the research focused on probing tritepene saponins, polysaccharides and their active functions.The roots of P. sibiricum Redoute were weighted for 25kg and cutted up. The 70% ethanol was 6 times as much as raw materials decocting 2h. The above experiments all repeated for 3 times. The ethanol extracts were extracted by petroleum ether and butanol . The butanol fraction was isolated by the column chromatography on macroporous resin AB-8, ODS and MCI with ethanol gradient elution and TLC. Two compounds were obtained.Their structures were identified by NMR spectroscopic analysis. Their structures were elucidated as 2β, 3β(OH)2 - (28→1) Glucoside - (6→1) Glucoside - (4→1) Rhamnoside - ursanal (asiaticoside) and 2β, 3β, 6β(OH)3 - (28→1) Glucoside - (6→1) Glucoside - (4→1) Rhamnoside - ursanal (madecassoside). The two compounds were isolated from roots of P. sibiricum Redoute and both were not reported.The crude polysaccharides were extracted 3 hours by hot water, and precipitated 3 times by ethanol, then washed several times with 100% ethanol. The experiment of polysaccharides on the hypoglycemic effects showed that polysaccharides from P. sibiricum Redoute had apparent functions of decreasing blood sugar to alloxan-induced hyperglycemic mice.The polysaccharides were purified by column chromatography on macroporous resin AB-8, and divided into three parts. Then part A was purified by column chromatography on DEAE-cellulose, Sephadex G-100 and HW-40. Moreover, TLC was used for the track examination. One polysaccharide product was obtained. Its structure was determined by GC and NMR spectroscopic analysis. Its main chain was linked by ketoses withβ-1,2 bond. Glucoses were linked byα- configuration in the form of side chain.