Investigation Of Deammonification Microorganism And Anammox In SBR Reactor

Accumulation of ammonia in water environment has induced serious deterioration to the quality of water environment. It is a problem that should be solved by using proper measure to prevent the deterioration without delay. Wastewater with high nitrogen concentrations and low C/N ratio are rather cost-intensive when treated with conventional concepts, as both the oxygen demand for nitrification and the demand for organic substrates for denitrification depend on the respective nitrogen concentration in the wastewater. It goes against the sustainable development of wastewater treatment. Recently a new process named as deammonification, which describes the oxidation of nearly 50% of the NH4+ to NO2- and subsequent conversion of NH4+ and NO2- to molecular nitrogen (N2 gas). The main advantages of these process is lower oxygen demand (up to 25% energy savings during aeration) and reduced 40% organic substrate requirements for heterotrophic denitrification, so it become the highlight in the biological de-nitrogen study.Based on the latest research findings on biological nitrogen removal in both domestic and abroad, the influence of inorganic carbon concentration, pH value and dissolved oxygen (DO) in deammonification was studied in a SBR reactor using anaerobic granulation sludge as the inoculation. In order to investigate the possibility of the granulation of deammonification bacteria .The experiment results showed that the pH value in the reaction system keep from 7.7 to 8.3 ,the temperature keep (35±1)oC and DO concentration be controlled at 0.5mg/L would plant the granulation of deammonification bacteria.In order to seed deammonification granulation sludge in an anaerobic sequencing batch reactor,the anaerobic ammonium-oxidized (ANAMMOX) was successful enrich at (35±1)oC after a 97-day cultivation, with the influent pH value ranging from 7.7 to 8.3. Experimental result indicate that with a hydraulic settling time of 3d and a volumetric loading rate ( calculated with the content of N ) of 0.1702 kg/(m3·d), the maximum removal of the total nitrogen respectively achieve 82.7%, while the maxi- -mum removals of NH4+-N and NO2--N respectively achieve 86.2% and 86%.The anaerobic ammonium oxidation is found to be the main reaction in an anaerobic sequencing batch reactor, which means that an anaerobic sequencing batch reactor is one of the useful tools to expediousness enrich the anaerobic ammonium-oxidized sludge.