| Functionalized gold composite materials as one of the branches in the fields of gold nanoparticles materials have a clearly advantage and broad application prospects, due to its good biocompatibility,large specific surface area and better conductive properties in the field of electrochemistry,especially in the fabrication of bio-electrochemical sensor.In this paper,four modified electrodes based on gold nanoparticles complex materials,Cyclodextrin-Poly(4-Aminothiophenol)-Gold Nanoparticles(CD-PAT-GNPs),Functional Ionic Liquid-Gold Nanoparticles(FIL-GNPs) and Functional Gold Nanoparticles(FGNPs) were fabricated and applied to study the direct electrochemistry and electrocatalysis of GOx and CAT.The investigations have some scientific signification for the exploration of new method in the synthesis of gold nanoparticles complex materials,the development of new electrochemical biosensors. The thesis was divided into four chapters and the author's main contribution as follows:The CD-PAT-GNPs was synthesized and Glucose Oxides/Cyclodextrin-Poly (4-Aminothiophenol)-Gold Nanoparticles/Carbon Ceramic Electrode(GOx/CD-PAT-GNPs/CCE) was fabricated and behavior of direct electrochemical of GOx and its electrocatalytic studies was studied.The results show that the modified electrode displayed a couple of stable and well-defined redox peaks with the formal potential(E?) of-0.488 V;and the direct electron transfer rate constant ks was 4.58 s-1 between GOx and modified electrode;the modified electrode plaly the role of electrocatalytic for glucose,and the steady-state current response increased linearly with glucose concentration from 2.5×10-4 mol·L-1 to 1.6×10-2 mol·L-1 with the detection limit of 9.0×10-5 mol·L-1.Chitosan-Catalase-Functional Ionic Liquid-Gold Nanoparticles/Carbon Ceramic EIectrode(CS/CAT/FIL-GNPs/CCE) was fabricated and the behavior of direct electrochemical CAT and its electrocatalytic studies was studied.The results show that the modified electrode displayed a couple of stable and well-defined redox peaks with the formal potential(E?) of-0.366 V;and the direct electron transfer rate constant ks was 3.12 s-1 between CAT and modified electrode,The apparent Michaelis constant Km was 1.15×10-5 mol·L-1;the modified electrode play the role of electrocatalytic for H2O2, and the steady-state current response increased linearly with concentration of H2O2 from 4.5×10-6 mol·L-1 to 2.5×10-5 mol·L-1 with the detection limit of 1.0×10-6 mol·L-1.Chitosan-Catalase-MultiwaU Carbon Nanotubes-Functionalized Ionic Liquid-Gold Nanoparticles-Glassy Carbon Electrode(CS/CAT/MWCNT-FIL-GNPs/GCE) was fabricated with method of mixing and dropping the CS,CAT,MWCNT and FIL-GNPs to the surface of GCE and the behavior of direct electrochemical CAT and its electrocatalytic was studied.The results show that the modified electrode displayed a couple of stable and well-defined redox peaks with the formal potential(E?) of-0.452 V;and the direct electron transfer rate constant ks was 5.56 s-1 between CAT and modified electrode,the apparent Michaelis constant Km was 1.15×10-5 mol·L-1;the modified electrode play the role of electrocatalytic for H2O2,and the steady-state current response increased linearly with concentration of H2O2 from 2.1×10-6 mol·L-1 to 2.5×10-5 mol·L-1 with the detection limit of 3.0×10-7 mol·L-1.Chitosan/Glucose Oxidase/Functional Gold Nanoparticles/Glassy Carbon Rlectrode(CS/GOx/FGNPs/GCE) was fabricated with method of mixing and dropping the CS,GOx and FGNPs to the surface of GCE and the behavior of direct electrochemical GOx and its electrocatalytic was studied.The results show that the modified electrode displayed a couple of stable and well-defined redox peaks with the formal potential(E?) of-0.452 V;and the direct electron transfer rate constant ks was 20.1 s-1 between GOx and modified electrode,the apparent Michaelis constant Km was 3.63×10-3 mol·L-1;the modified electrode play the role of electrocatalytic for glucose oxidation,and the steady-state current response increased linearly with glucose concentration from 5.0×10-4 mol·L-1 to 1.5×10-2 mol·L-1 with the detection limit of 1.0×10-4 mol·L-1.
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