| Ion Chromatography is a branch of the High Performance Liquid Chromatography and it is a liquid chromatography method for the determination of the anions and cations.IC has good selectivity, high sensitivity,rapidity,simplicity and can detect various components simultaneously for the determination of the common cations and anions,the organic acids and amines which were difficult to detected by other analytical methods.Based on these advantages,since 1975 IC has a rapid development and has been widly used in many fields,such as environmental monitoring,electric power,semiconductor,foods,petroleum and chemical industry,medical and health,biochemistry and so on.At the meantime there are already in dozens of IC methods become the standard method for the authority.Ground on the state of ionization in alkali and the electrochemical activity of the sugars,Roxklin had reported the method that use the anion exchange column and pulsed amperemetric detection to determined the polysaccharides in 1983.Due to it's simply manipulate and accuratly results,using IC for determining sugars deserves people's concern and study.The thesis consists of two parts.One is a review related to the application and the extraction of the polysaccharides,and makes an evaluation on the development of the IC detection technology and the other fields'detectation in recent ten years.The other is a research report which is composed of four components as follows.First of all,do the research on the extraction and the determination of glucose and mannose which are the two kinds of tea polysaccharides in green tea with IC technology. A fast,simple method was established and the best extracte condition was chosen at the same time. Using the warm water to distill the green tea and ultrasound compnent with the HAc to hydrolyze the tea polysaccharides,take the METROSEP CARB1(150×4.0 mm) anion exchange column as the chromatograph IC separation,at 32℃using the 6.0mmol/L NaOH as the mobile phase.The glucose and the mannose were totally separated and determined.Consideration of the top research,applying this system to the four kinds of teas,improving the condition,inlarge the application from the only two monosaccharides to four kinds of monosaccharides,after that good results were be gotten.This method broke the usual method that need gradient mobile phase,only need one fixed flow liquid which can easily separated the polysaccharides.Then apply this method to determine the polysaccharides which are distracted from the mushroom and the CommonYan Rhizome,good results were gotten and the method was be optimized.But in these researches,there are also some disadvantages,such as the determination of the fructose,clearly manifest as the low sensitivity and the long determine time that cost.For the development of the method,go on to improve the condition,and a fast,accurate method for determining fructose by IC was established with a high accuracy and low detection limite.1.The studies of the extractionand and the Ion Chromatography determination of tea polysaccharides in Green TeaA method for determine tea polysaccharides of glucose and mannose in green tea by high performance anion exchange with pulsed amperemetric detection was established,based on the different distill methods.The chromatograph separation was established on METROSEP CARB 1 (150×4.0 mm) anion exchange column,using 6.0 mmol/L NaOH at 1.0 mL/min in flow rate.The total analysis time is 35 min.The detection limits of 0.125 mg/L～2.0 mg/L(20μL injection,using the peak height for the quantitative analysis) were achieved under the optimal conditions.The RSDs of peak height for the seven consecutive determinations of 20 mg/L glucose and mannose were 5.79%and 14.15%respectively,and the RSDs of reserve time were 2.08%and 2.19%.The recovery percentages of the sample detection were 94.9%～97.5%.Glucose and mannose were be determined fast and effectively,at the meantime,the best distill condition was be chosen.2.The determination of tea polysaccharides with Ion ChromatographyA method for the determination of tea polysaccharides of galactose,glucose,mannose and fructose by high performance anion exchange with pulsed amperemetric detection was established.The chromatograph separation was established on METROSEP CARB 1(150×4.0 mm) anion exchange column,using 6.0 mmol/L NaOH at 1.0 mL/min in flow rate and at 32℃.The total analysis time is 45 min.The detection limits of 0.125 mg/L～2.0 mg/L(20μL injection,using the peak height for the quantitative analysis) were achieved under the optimal conditions.The RSDs of peak height for the seven consecutive determinations of 20 mg/L galactose,glucose,mannose and 30 mg/L fructose were 5.82%～14.19%,and the RSDs of reserve time were 2.03%～2.26%.The recovery percentages of the sample detection were 91.8%～99.3%.Four kinds of tea polysaccharides were fast and effectively determined,and the results of the determination were satisfying.3.Studies of the determination of the lentinan and the dioscoreaopposita polysaccharides by ICThis article applies the used of the IC technology to the fungus and Chinese traditional medicines field and a fast determining method has been established.The chromatograph separation was established METROSEP CARB 1(150×4.0 mm) anion exchange column,with pulsed amperemetric detection.Using 7.0 mmol/L NaOH at 1.0 mL/min in flow rate.The total analysis time is 35min.The detection limits was 0.125～1.0 mg/L(20μL injection,using the peak height for the quantitative analysis) were achieved under the optimal conditions.The RSDs of peak height for six consecutive determinations of 30 mg/L standard sample were 1.79%～3.52%,and the RSDs of reserve time were 3.07%～3.29%.The recovery percentages of the sample detection were 96.3%～99.5%.The system was applied for the determination of lentinan and dioscoreaopposita,good separation and high detection sensitivity were achieved,and the results of the determination were satisfying.4.The determination of the fructose by ICA method for determination of fructose in hydrolyzing polysaccharides by high performance anion exchange with pulsed amperemetric detection was established.And the accuracy that use the peak height and peak area for quantitative analysis were discussed.The chromatograph separation was established on METROSEP CAR 1(150×4.0 mm) anion exchange column,using 100 mmol/L NaOH at 1.0 mL/min in flow rate.The total analysis time is 13min.The detection limits of 0.4 mg/L(20μL injection) was achieved under the optimal conditions.The RSDs of peak height for seven consecutive determination of 10 mg/L fructose was 1.64%,the RSDs of peak area 3.43%,and the RSDs of reserve time 0.36%.The recovery percentages of sample detection were 94.6%～96.1%(using peak height) and 93.2%～95.6%(using peak area).Three kinds of tea polysaccharides were fast and effectively determined,and the results of the determination were satisfying.
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