Study On Phosphorylation And Succinylation Of Korean Pine Seed Protein

Korean pine is a species of high economic value, but also is one of China's precious tree species. The center of Korean pine natural distribution is in Northeast China. Korean pine kernel contains higher protein. In this paper, Korean pine is as raw material, sodium tripolyphosphate and amber acid without were used in different conditions of molecular modification. The main results are as follows:1. Homogenate with the use of aqueous extraction of crude protein, The best conditions were as follows: homogeneous broken 4min, liquid ratio 1:25, pH value of 9.0, extraction time 60min, temperature 35°C. Under these conditions extraction rate of Korean pine protein isolate was up to 61.03%. With the traditional "acid alkaline extraction" method, Korean pine nut protein isolate can significantly increase the extraction rate.2. In this study, sodium tripolyphosphate (STP) as a phosphorylation modification reagents, using response surface analysis to optimize the process parameters of the modified Korean pine nut protein isolate of the solubility. The study found: reaction temperature, pH value, STP concentration and reaction time affect protein solubility of Korean pine protein isolate, followed by the impact: STP concentration> pH value> reaction time> the reaction temperature. The best conditions of phosphorylated modified Korean pine protein isolate dissolved are: reaction temperature 45°C, pH value of 8.5, STP concentration of 7%, the reaction time was 75min. Under these conditions solubility of korean pine protein isolate was up to80.2%. With phosphoric acid solubility of the pre-modified (26.9%) compared to 198%improved.3. Modification of the phosphorylation of Korean pine protein isolate the emulsion was found: reaction temperature, reaction time and concentration of STP on impact of Korean pine protein isolate was as follows: reaction time> STP concentration> temperature. The best emulsification conditions of phosphorylation modified Korean pine protein isolate: pH value8, reaction temperature 46°C, STP concentration 8%, the reaction time was 65min. Under these conditions emulsification of Korean pine protein isolate was up to 61.03%. And phosphorylation of the pre-modified emulsion degrees (23%) compared to 165% improved. With this, we can see that solubility and emulsifying of phosphorylation modified Korean pine protein isolate improved.4. In this study, as the succinate succinic anhydride acylating reagent modified by response surface analysis to optimize the process parameters of the modified Korean pine protein isolate solubility and emulsification. The study found: Amber acylation of the modified Korean pine protein isolate analysis of the impact on the solubility. We can find: reaction time> the amount of succinyl-anhydride> reaction temperature. Amber acylation and modified Korean pine protein isolate of emulsification for the factors: the amount of succinyl-anhydride> reaction time> the reaction temperature.5.According to single factor experiments and response surface method optimization of Korean pine protein isolate acylation amber modified ,solubility and emulsifying effects of the best conditions are as follows: the pH value of 8, korean pine protein isolate mass fraction of 10% , the reaction temperature 46°C, the amount of succinyl-anhydride 15%, the reaction time was 40min. Under these conditions solubility of Korean pine protein isolate was up to 78.9% . reaction temperature 46°C, the amount of succinyl-anhydride 16%, the reaction time was 30min. Under these conditions emulsification of korean pine protein isolate was up to 56.96%.6. Solubility and emulsifying capacity of phosphorylation modified are higher than the amber modified of Korean pine protein isolate. But the amber acylation modified texture features are better than phosphorylation modified. Considered, selected phosphorylation as modified Korean pine protein isolate is superior to anhydrous succinic acid reagent.