Determination Of Agents In Fruits, Vegetables And Cosmetics By Using Second-order Calibration Methods Coupled With Three-dimensional Fluorescence

In the introductory part of this thesis, the background and main research contents of chemometrics are illustrated. The reason why the second-order calibration methods in chemometrics are gaining widespread acceptance among the analytical community is then mainly discussed. Finally, we overview their applications in the analysis of the components of interest in complex systems.Chapter 2 proposes a novel approach for direct quantitative analysis of thiabendazole in orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternating trilinear decomposition (ATLD) and the alternating normalization-weighted error (ANWE) respectively. The experimental results demonstrate that both methods have been satisfactorily applied to the determination of thiabendazole content in orange extract, and the performance of ANWE was slightly better than that of ATLD.Chapter 3 proposes a novel approach for direct quantitative analysis of bitertanol in banana extract by the incorporation of three-dimensional fluorescence with second-order calibration methodologies based on the alternating trilinear decomposition (ATLD),the alternating fitting residue (AFR) and the self-weighted alternating trilinear decomposition (SWATLD) algorithms respectively. The experimental results demonstrate that these three algorithms have been successfully applied to determine the content of bitertanol in banana extract.In Chapter 4, the feasibility of employing EEM fluorescence with ATLD and ANWE algorithms to simultaneously determine fluoranthene and chrysene contents in the lettuce extract has been discussed. The experimental results demonstrate that both algorithms have been successfully applied to the determination of chrysene content in lettuce extract, and both can also be used to analyze fluoranthene in lettuce extract, and the performance of ANWE was slightly better than that of ATLD for both compounds analyzed in such a system according to the elliptical joint confidence region test.In Chapter 5, a sensitive excitation-emission ?uorescence method was proposed to determine testosterone propionate (TP) in four cosmetics with the aid of second-order calibration methods based on the parallel factor analysis (PARAFAC), the alternating penalty trilinear decomposition (APTLD) and the self-weighted alternating trilinear decomposition (SWATLD) algorithms respectively. TP can be transformed into a highly ?uorescent derivative through oxidation reaction with concentrated sulfuric acid (H2SO4). The satisfactory results from these cosmetics used in the analysis demonstrate that this method can be used to determine the TP content of cosmetics.