Preparation Of Chelate Of Proteolysis From Harengula Zunasi Bleeker's Leftover And Its Biological Character Study

In this paper, the ions binding conditions of hydrolysate by compound enzymes from Harengula Zunasi Bleeker's leftover and its antioxidant activity, antibacterial activity were studied. This work may provide a new way and theoretical basis for high-value utilization of Harengula Zunasi Bleeker.This study includes five parts of content as follows:1. The effect of hydrolyzed time on DH in the conditions of fatty or defatty by compound enzymes.2. The effect of different DH, pH, temperature and time on the chelate ratio of Fe²⁺ and Harengula Zunasi Bleeker enzymatic hydrolysate.3. Infrared spectrum analysis of chelate structure.4. Primary research on antioxidant activity of peptides chelatingFe²⁺.5. Primary research on antibacterial activity of peptides chelatingFe²⁺. And the results showed that1)DH was 5%, 10%, 15%, 20% when hydrolyzed time was 5h, 11h, 16.5h, 24.8h in the conditions of defatty or 2h, 4h, 10h, 23h in the conditions of fatty.2) The optimal chelatingFe²⁺ ratio was 94.15 in the condition of DH 5%, pH 7, temperature 20℃, time 30min.3) The peptides could chelate metal ions and they combined with monodentate covalent bond by infrared spectrum analysis.4) The antioxidant activity of peptides chelatingFe²⁺ was obviously after 50% ethanol precipitation and was corresponding to 92.35% of tocopherol as control.5) With Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella as research object, the antibacterial activity of peptides chelatingFe²⁺ had been determined by the method of Oxford Cup two layer plating in vitro, respectively. And inhibitory effect of the chelatingFe²⁺ by fractional precipitation with 80% ethanol to Bacillus subtilis, Staphylococcus aureus was relatively obvious.