Study On Interaction Of Different Excellent Strains In The Process Of Degrading Hydrocarbon Pollutants

Hydrocarbon pollutants commonly found in the environment,because of its low water solubility and biological toxicity,in particular polycyclic aromatic hydrocarbons has strong carcinogenic that is a very serious threat on the environment and human health and make hydrocarbon pollution of the environment has become urgent one of the problems.First,this paper summary of the pollution sources and status of hydrocarbons, research progress of microbial degradation hydrocarbon substances,and research issues that exist in a more comprehensive overview.Then,the excellent strains degrade the petroleum hydrocarbons and the polycyclic aromatic hydrocarbons,which selection from the pollution of the environment.Research on the relationship between cell surface hydrophobicity and the degradability of hydrocarbon.And choose to use three excellent hydrocarbon degrading strains,composed of different combinations,experimental studies the rate of hydrocarbon degradation,the trends of relative cell number in the process of the degradation,pH value changes in degradation of fluid,trends of the cell surface hydrophobicity.Through experimental research,the main results are as follows:(1) It obtained eleven hydrocarbon degrading strains and seven polycyclic aromatic hydrocarbons degrading strains,form the indigenous mixed bacteria of contaminated soil and sediment in the coking wastewater.After six days,the rate of petroleum hydrocarbons degradation of four hydrocarbon degrading strains were more than 35%, and the average degradation rates of seven polycyclic aromatic hydrocarbons degrading strains for Anthracene,Phenanthrene and pyrene were 37%,83%,31%.(2) The Surface hydrophobicity of the strains is closely related to the hydrocarbon degradation rate.In general,the greater surface hydrophobicity of strains,the better hydrocarbon degradation rate.(3) Single strain S3,S6,S9 in six days of petroleum hydrocarbon degradation rates were 45.3%,28.6%,42.3%;S3 and S9 mixture of petroleum hydrocarbon degradation rate was 55.4%,hybrid strains to promote the degradation of petroleum hydrocarbons; S6 and S9 mixture of petroleum hydrocarbon degradation rate was only 35.5%,hybrid strains is not conducive to degradation of petroleum hydrocarbons;S3,S6 and S9 mixture of petroleum hydrocarbon degradation rate was 48.8%,hybrid strains effect on the degradation of petroleum hydrocarbons rather more a single strain,strains mixed with different combinations,such as the promotion of a clear S3 + S9,the degradation rate was 55.4%;the effect of weakening such as the S6 + S9,the degradation rate was 35.5%;also have little effect,such as S3 + S6 + S9,the degradation rate was 48.8%.(4) The capacity degradation of polycyclic five kinds of PAHs by polycyclic aromatic hydrocarbons strains,smaller of the order:fluorene,phenanthrene,anthracene, fluoranthene,pyrene.Strains H3 and H6 strain combinations,competitive relationship between species.Mixed group of H3 and H6 are complementary role catabolism.Mixed group of H3 and H7 is no significant interaction effect;Mixed group of H3,H6 and H7, there is a co-coordinated response in prophase,mutual inhibition in late stage.(5) In degradation process of excellent strains to degrade petroleum hydrocarbons and polycyclic aromatic hydrocarbons,that a single bacteria cell number and cell surface hydrophobicity of the rate of change,that were explain the trends of degradation rate of mixed bacteria group that can be used as an selection experiment method of a good mixture agents.Experimental results obtained form this paper,providing a reference data and methods for further study on the interaction of different strains in biodegrading hydrocarbons and microbial agents to provide.